Version 1
: Received: 20 December 2023 / Approved: 20 December 2023 / Online: 20 December 2023 (12:08:01 CET)
How to cite:
Liu, X.; Yin, C.; Zhou, C.; Wang, X. Comprehensive Analysis of Transcriptomic and Proteomic Revealed Nitrogen Deficit Effects on Photosynthesis and Carbon Metabolism of Wheat. Preprints2023, 2023121557. https://doi.org/10.20944/preprints202312.1557.v1
Liu, X.; Yin, C.; Zhou, C.; Wang, X. Comprehensive Analysis of Transcriptomic and Proteomic Revealed Nitrogen Deficit Effects on Photosynthesis and Carbon Metabolism of Wheat. Preprints 2023, 2023121557. https://doi.org/10.20944/preprints202312.1557.v1
Liu, X.; Yin, C.; Zhou, C.; Wang, X. Comprehensive Analysis of Transcriptomic and Proteomic Revealed Nitrogen Deficit Effects on Photosynthesis and Carbon Metabolism of Wheat. Preprints2023, 2023121557. https://doi.org/10.20944/preprints202312.1557.v1
APA Style
Liu, X., Yin, C., Zhou, C., & Wang, X. (2023). Comprehensive Analysis of Transcriptomic and Proteomic Revealed Nitrogen Deficit Effects on Photosynthesis and Carbon Metabolism of Wheat. Preprints. https://doi.org/10.20944/preprints202312.1557.v1
Chicago/Turabian Style
Liu, X., Changan Zhou and Xinyu Wang. 2023 "Comprehensive Analysis of Transcriptomic and Proteomic Revealed Nitrogen Deficit Effects on Photosynthesis and Carbon Metabolism of Wheat" Preprints. https://doi.org/10.20944/preprints202312.1557.v1
Abstract
Excessive nitrogen application has become a hidden danger threatening the sustainable development of agriculture. Exploring the potential genes will help to improve the nitrogen availability of wheat through molecular breeding. We therefore conducted wheat transcriptomics and proteomics researches under complete culture medium without nitrogen (N0) and complete culture medium with nitrogen (N1). The results showed that, the nitrogen deficit decreased the fresh weight, N content and net photosynthetic rate of winter wheat. 3949 and 2675 differentially expressed genes (DEGs) were identified in the shoot and root, respectively, while 164 and 129 differentially expressed proteins (DEPs) were identified in the shoot and root respectively. 9 genes in shoot and 26 genes in root showed the same trends in RNA and protein levels. 5 genes in shoot and 4 genes in root showed the opposite trends in RNA and protein levels. Pathway enrichment of wheat transcriptome and proteome under nitrogen deficit proposed 3 candidate genes related to photosynthesis in shoot, 4 candidate genes related to carbon metabolism in shoot, and 12 candidate genes related to carbon metabolism in root. Meanwhile, the chromosomal and subcellular localization of these genes were listed, which will provide reference for improving nitrogen availability through molecular breeding.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
