Version 1
: Received: 13 December 2023 / Approved: 14 December 2023 / Online: 14 December 2023 (10:07:30 CET)
How to cite:
Stefanov, I.S. ChAT Immunoreactive Structures in the Porcine Extrahepatic Bile Ducts. Preprints2023, 2023121036. https://doi.org/10.20944/preprints202312.1036.v1
Stefanov, I.S. ChAT Immunoreactive Structures in the Porcine Extrahepatic Bile Ducts. Preprints 2023, 2023121036. https://doi.org/10.20944/preprints202312.1036.v1
Stefanov, I.S. ChAT Immunoreactive Structures in the Porcine Extrahepatic Bile Ducts. Preprints2023, 2023121036. https://doi.org/10.20944/preprints202312.1036.v1
APA Style
Stefanov, I.S. (2023). ChAT Immunoreactive Structures in the Porcine Extrahepatic Bile Ducts. Preprints. https://doi.org/10.20944/preprints202312.1036.v1
Chicago/Turabian Style
Stefanov, I.S. 2023 "ChAT Immunoreactive Structures in the Porcine Extrahepatic Bile Ducts" Preprints. https://doi.org/10.20944/preprints202312.1036.v1
Abstract
Background: In the domestic pig, neural and non-neuronal cholinergic systems in the gallbladder and extrahepatic bile ducts need a detailed morphological study to identify the type and localization of their structural components. We aimed to quantitatively analyze biliary cholinergic ganglia and nerves as well as non-neuronal cholinergic cells; Methods: Immunohistochemical detection of choline acetyltransferase expression was performed to define cholinergic nerve and non-neuronal structures in gallbladder and extrahepatic bile ducts; Results: The number and dimensions of cholinergic ganglia, perikaryons and nerves forming ganglionic nerve plexuses in the propria, muscular and subserosal layers was estimated. The immunoexpression of choline acetyltransferase in biliary glandular cells and mast cells was also detected and analyzed. Conclusions: Qualitative and quantitative analysis of choline acetyltransferase positive nerves, ganglionic and non-ganglionic nerve plexuses of acetylcholine supplemented the morphological knowledge of the cholinergic innervation of the extrahepatic biliary pathways and non-neuronal sources: glandular cholinergic cells and cholinergic-type mast cells. Original data of the number and dimensions of intramural nerves, mast cells, ganglia and ganglion perikaryons could be used as reference values in the diagnosis of neuropathological processes in biliary pathways. Clarifying the precise mechanisms of gallbladder smooth muscle contractility will contribute to the improvement of therapeutic strategies to prevent biliary stasis and biliary disorders.
Keywords
mast cells; ganglia; choline acetyltransferase; tryptase; bile ducts; pigs
Subject
Medicine and Pharmacology, Anatomy and Physiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
