Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Evaluation of Structural Characteristics, Cytotoxicity and Im-Munomodulation Activity In Vitro and In Vivo of Polysaccharide from Ficus carica L.

Lin Ye
ORCID logo ,
Qin-Qiu Zhang
,
Shang Lin
,
Qing Zhang
ORCID logo ,
Jing Yan
,
Ding-Tao Wu
,
Shu-Xiang Liu
ORCID logo ,
Wen Qin
*
Version 1 : Received: 11 December 2023 / Approved: 12 December 2023 / Online: 12 December 2023 (10:23:35 CET)

A peer-reviewed article of this Preprint also exists.

Ye, L.; Zhang, Q.-Q.; Lin, S.; Zhang, Q.; Yan, J.; Wu, D.-T.; Liu, S.-X.; Qin, W. A Polysaccharide from Ficus carica L. Exerts Immunomodulatory Activity in Both In Vitro and In Vivo Experimental Models. Foods 2024, 13, 195. Ye, L.; Zhang, Q.-Q.; Lin, S.; Zhang, Q.; Yan, J.; Wu, D.-T.; Liu, S.-X.; Qin, W. A Polysaccharide from Ficus carica L. Exerts Immunomodulatory Activity in Both In Vitro and In Vivo Experimental Models. Foods 2024, 13, 195.

Abstract

Polysaccharide from Ficus carica L. (FCP) exert multiple biological activities, as a biological macromolecule, the available knowledge about the specific structures and mechanisms of biological activity of purified 'Brunswick' fig polysaccharide is currently limited. In the present study, by chemical purification and characteristics identified via specific instruments, and then the impact of FCP on the immunomodulation activity in vitro and in vivo was examined. Structural char-acteristics showed that the molecular weight of the FCP was determined to be 127.5 kDa, the primary monosaccharides present in the FCP were galacturonic acid (GalA), arabinose (Ara), galactose (Gal), rhamnose (Rha), glucose (Glc), and xylose (Xyl) at a ratio of 0.321:0.287:0.269:0.091:0.013:0.011. Based on the investigation of in vitro immunomodulatory ac-tivity, FCP was found to stimulate the production of NO, TNF-α, and IL-6 and increased the pi-nocytic activity of macrophages. Further analysis revealed that FCP activated macrophages by interacting with toll-like receptor 4 (TLR4). Moreover, the in vivo test results indicate that FCP showed significant increase serum pro-inflammatory factors in immunosuppressed mice. Overall, this study suggests that FCP has the potential to be utilized as a novel immunomodulator in the pharmaceutical and functional food industries.

Keywords

Ficus carica L.; Polysaccharide; Structural characteristics; Cytotoxicity; Immunomodulatory activity

Subject

Biology and Life Sciences, Food Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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