Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Temporal Trend of the SARS-CoV-2 Omicron Variant and Res-Piratory Syncytial Virus in the Nasal Cavity and Accuracy of the Newly Developed Antigen-Detecting Rapid Diagnostic Test

Daisuke Tamura
* ORCID logo ,
Yuji Morisawa
,
Takashi Mato
,
Shin Nunomiya
ORCID logo ,
Masaki Yoshihiro
,
Yuta Maehara
,
Shizuka Ito
,
Yasushi Ochiai
,
Hirokazu Yamagishi
,
Toshihiro Tajima
,
Takanori Yamagata
,
Hitoshi Osaka
Version 1 : Received: 5 December 2023 / Approved: 6 December 2023 / Online: 6 December 2023 (07:38:00 CET)

A peer-reviewed article of this Preprint also exists.

Tamura, D.; Morisawa, Y.; Mato, T.; Nunomiya, S.; Yoshihiro, M.; Maehara, Y.; Ito, S.; Ochiai, Y.; Yamagishi, H.; Tajima, T.; Yamagata, T.; Osaka, H. Temporal Trend of the SARS-CoV-2 Omicron Variant and RSV in the Nasal Cavity and Accuracy of the Newly Developed Antigen-Detecting Rapid Diagnostic Test. Diagnostics 2024, 14, 119. Tamura, D.; Morisawa, Y.; Mato, T.; Nunomiya, S.; Yoshihiro, M.; Maehara, Y.; Ito, S.; Ochiai, Y.; Yamagishi, H.; Tajima, T.; Yamagata, T.; Osaka, H. Temporal Trend of the SARS-CoV-2 Omicron Variant and RSV in the Nasal Cavity and Accuracy of the Newly Developed Antigen-Detecting Rapid Diagnostic Test. Diagnostics 2024, 14, 119.

Abstract

To analyze the viral titers of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and respiratory syncytial virus (RSV) at the anterior nasal site (ANS) and nasopharyngeal site (NS), evaluate their virological dynamics, and validate the usefulness of a newly developed two antigen-detecting rapid antigen diagnostic test (Ag-RDT) that simultaneously detects SARS-CoV-2 and RSV using clinical specimens. This study included 195 asymptomatic to severely ill patients. Overall, 668 specimens were collected simultaneously from the ANS and NS. The cycle threshold (Ct) values calculated from real-time polymerase chain reaction were used to analyze temporal changes in viral load and evaluate the sensitivity and specificity of the Ag-RDT. The mean Ct values for SARS-CoV-2-positive, ANS, and NS specimens were 28.8, 28.9, and 28.7, respectively. The mean Ct values for RSV-positive, ANS, and NS specimens were 28.7, 28.8, and 28.6, respectively. SARS-CoV-2 and RSV showed the same trend in viral load, although the viral load of NS was higher than that of ANS. The sensitivity and specificity of the newly developed Ag-RDT were excellent in specimens collected up to 10 days after the onset of SARS-CoV-2 infection and up to 6 days after the onset of RSV infection.

Keywords

Omicron; rapid diagnostic test; RT-PCR; RSV; SARS-CoV-2; viral antigens

Subject

Medicine and Pharmacology, Clinical Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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