Version 1
: Received: 29 November 2023 / Approved: 30 November 2023 / Online: 30 November 2023 (08:27:58 CET)
How to cite:
Fu, J.; Hao, A.; Zhang, X.; Wang, D.; Liu, W.; Zhang, C.; Zhao, D.; Zhao, Z.; Zhang, L.; Sun, F. Analysis of LncRNA and mRNA Expression Profiles in Skin Tissues of Super Merino Sheep and Small-Tailed Han Sheep. Preprints2023, 2023111959. https://doi.org/10.20944/preprints202311.1959.v1
Fu, J.; Hao, A.; Zhang, X.; Wang, D.; Liu, W.; Zhang, C.; Zhao, D.; Zhao, Z.; Zhang, L.; Sun, F. Analysis of LncRNA and mRNA Expression Profiles in Skin Tissues of Super Merino Sheep and Small-Tailed Han Sheep. Preprints 2023, 2023111959. https://doi.org/10.20944/preprints202311.1959.v1
Fu, J.; Hao, A.; Zhang, X.; Wang, D.; Liu, W.; Zhang, C.; Zhao, D.; Zhao, Z.; Zhang, L.; Sun, F. Analysis of LncRNA and mRNA Expression Profiles in Skin Tissues of Super Merino Sheep and Small-Tailed Han Sheep. Preprints2023, 2023111959. https://doi.org/10.20944/preprints202311.1959.v1
APA Style
Fu, J., Hao, A., Zhang, X., Wang, D., Liu, W., Zhang, C., Zhao, D., Zhao, Z., Zhang, L., & Sun, F. (2023). Analysis of LncRNA and mRNA Expression Profiles in Skin Tissues of Super Merino Sheep and Small-Tailed Han Sheep. Preprints. https://doi.org/10.20944/preprints202311.1959.v1
Chicago/Turabian Style
Fu, J., Lichun Zhang and Fuliang Sun. 2023 "Analysis of LncRNA and mRNA Expression Profiles in Skin Tissues of Super Merino Sheep and Small-Tailed Han Sheep" Preprints. https://doi.org/10.20944/preprints202311.1959.v1
Abstract
Wool quality and yield are important economic traits of livestock. In this study, the skin tissues of the upper scapula of Super Merino sheep (SM) and Small-Tailed Han sheep (STH) during the growing period were studied. Differentially expressed (DE) long non-coding RNAs (lncRNAs) and messenger RNAs (mRNA) were identified via histopathological observation and high-throughput RNA sequencing. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to analyze differentially expressed genes. Reverse transcription quantitative Polymerase Chain Reaction (RT-qPCR) was used to verify nine selected DE lncRNAs and five DE mRNAs. Significant differences were found in the expression levels of LOC114113396 (KRTAP15-1), CRABP1, KRT10, KRTAP1-3, KRTAP4.3, KRT2, FGF1, IGF-1, and KIT in SM and STH skin tissues. These results indicate that these mRNAs may play an important role in the growth and development of hair follicles and the regulation of hair fiber traits. Differentially expressed lncRNA target genes and mRNAs were mainly enriched in biological processes and pathways related to skin development and fiber traits, which is consistent with previous studies. LncRNA MSTRG.9225.1, MSTRG.124320.1, MSTRG.96951.1, MSTRG.91685.1, MSTRG.122229.1, MSTRG.89255.1, MSTRG.98769.1, and MSTRG.84658.1 may indirectly participate in the growth and development of sheep hair follicles and the regulation of hair fiber diameter by regulating the expression of their respective target genes. These candidate lncRNAs and mRNAs may provide a basis for the study of sheep hair follicle growth and development and hair fiber traits.
Keywords
anagen; lncRNA; super merino sheep; small-tailed han sheep; RNA sequencing
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
