Alex-Asaolu, A.; Uba, A.; Birnin-Yauri, U.A.; Yusuf, A.J. Isolation and Characterization of Antimicrobial Constituent(s) from the Stem of Cissus populnea Guill. & Perr.. Drugs Drug Candidates2024, 3, 172-183.
Alex-Asaolu, A.; Uba, A.; Birnin-Yauri, U.A.; Yusuf, A.J. Isolation and Characterization of Antimicrobial Constituent(s) from the Stem of Cissus populnea Guill. & Perr.. Drugs Drug Candidates 2024, 3, 172-183.
Alex-Asaolu, A.; Uba, A.; Birnin-Yauri, U.A.; Yusuf, A.J. Isolation and Characterization of Antimicrobial Constituent(s) from the Stem of Cissus populnea Guill. & Perr.. Drugs Drug Candidates2024, 3, 172-183.
Alex-Asaolu, A.; Uba, A.; Birnin-Yauri, U.A.; Yusuf, A.J. Isolation and Characterization of Antimicrobial Constituent(s) from the Stem of Cissus populnea Guill. & Perr.. Drugs Drug Candidates 2024, 3, 172-183.
Abstract
Cissus populnea Guill. & Perr. (Vitaceae) is used in traditional medicine to treat microbial infec-tions, venereal diseases, and infertility, among others. The aim of the research is to isolate and characterize the antimicrobial constituent(s) from the stem of C. populnea. The n-butanol fraction of C. populnea being most active was subjected to silica gel column chromatography which led to the isolation of a white solid and white crystalline substances coded compounds C1 and C4C5, respectively. Spectral analysis (1D & 2D-NMR) of the isolated compounds and comparison with literature data indicated C1 to be Bis-(2-ethyloctyl)-phthalate and C4C5 to be a mixture of stig-masterol and β-sitosterol; C4C5 exhibited zone of inhibition ranging from 24 – 29 mm against the test organisms with Candida albicans being the most sensitive organism while Trichophyton rubrum was the least sensitive organism. The standard drugs, Ciprofloxacin had 27 – 37 mm while Flu-conazole and Fulcin exhibited zone of inhibition ranging from 34 mm and 29 – 32 mm, respec-tively. The MIC and MBC/MFC values for C4C5 ranges from 12.5 – 25.0 µg/cm3 and 25.0 – 50.0 µg/cm3 against MRSA, S. aureus, E. coli, C. albicans, T. rubrum, and T. mentagrophyte, respectively. In conclusion, three bioactive compounds were identified for the first time from the stem of C. populnea
Medicine and Pharmacology, Complementary and Alternative Medicine
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
