Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Maximising the Recovery of Phenolic Antioxidants from Wild Strawberry (Fragaria vesca) Leaves Using Microwave-Assisted Extraction and Accelerated Solvent Extraction

Petra Terpinc
* ,
Erika Dobroslavić
ORCID logo ,
Ivona Elez Garofulić
,
Maja Repajić
ORCID logo ,
Ena Cegledi
ORCID logo ,
Ana Dobrinčić
ORCID logo ,
Sandra Pedisić
ORCID logo ,
Branka Levaj
* ORCID logo
Version 1 : Received: 16 November 2023 / Approved: 17 November 2023 / Online: 17 November 2023 (15:29:30 CET)

A peer-reviewed article of this Preprint also exists.

Terpinc, P.; Dobroslavić, E.; Garofulić, I.E.; Repajić, M.; Cegledi, E.; Dobrinčić, A.; Pedisić, S.; Levaj, B. Maximizing the Recovery of Phenolic Antioxidants from Wild Strawberry (Fragaria vesca) Leaves Using Microwave-Assisted Extraction and Accelerated Solvent Extraction. Processes 2023, 11, 3378. Terpinc, P.; Dobroslavić, E.; Garofulić, I.E.; Repajić, M.; Cegledi, E.; Dobrinčić, A.; Pedisić, S.; Levaj, B. Maximizing the Recovery of Phenolic Antioxidants from Wild Strawberry (Fragaria vesca) Leaves Using Microwave-Assisted Extraction and Accelerated Solvent Extraction. Processes 2023, 11, 3378.

Abstract

Wild strawberry (Fragaria vesca L.) leaves possess diverse antioxidant properties, mainly due to the different classes of phenolics. In this study, microwave-assisted extraction (MAE) and accelerated solvent extraction (ASE) of wild strawberry phenolics were carried out to understand the impact of extraction temperature, extraction time and solvent-to-sample ratio (SSR) on the quantitative and qualitative properties of the obtained extracts. The highest total phenolic content (8027 mg GA/100 g DW), as well the highest DPPH• antiradical activity (903 mol TE/g DW) was obtained with ASE at 150 °C, static time 5 min and SSR of 40:1; while the highest ABTS•+ antiradical activity (681 mol TE/g DW,) and FRAP (2389 mol TE/g DW) was obtained with MAE after 5 min at 80 °C and a SSR of 40:1. The correlation analysis showed that phenolics largely contributed to the antioxidant properties of the studied MAE extracts, while correlation was not found in ASE extracts. A total of 54 different phenolics were identified by UPLC/MS-MS in MAE and ASE extracts obtained at optimal extraction conditions. MAE extract was shown to have a higher content of phenolic acids (40%), but lower content of proanthocyanidins (88%), flavonols (29%), flavan-3-ols (50%) and flavones (39%) than ASE extract.

Keywords

wild strawberry leaves; microwave-assisted extraction; accelerated solvent extraction; phenolic profile; antioxidant activity

Subject

Biology and Life Sciences, Food Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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