Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Cx3Mab-4: a Novel Anti-mouse CXCR3 Monoclonal Antibody for Flow Cytometry

Tsunenori Ouchida
ORCID logo ,
Yu Isoda
,
Tomohiro Tanaka
,
Mika K Kaneko
,
Hiroyuki Suzuki
* ORCID logo ,
Yukinari Kato
* ORCID logo
Version 1 : Received: 13 November 2023 / Approved: 13 November 2023 / Online: 14 November 2023 (05:37:03 CET)

A peer-reviewed article of this Preprint also exists.

Ouchida, T., Isoda, Y., Tanaka, T., Kaneko, M. K., Suzuki, H., & Kato, Y. (2023). Cx3Mab-4: a Novel Anti-mouse CXCR3 Monoclonal Antibody for Flow Cytometry (preprint). Ouchida, T., Isoda, Y., Tanaka, T., Kaneko, M. K., Suzuki, H., & Kato, Y. (2023). Cx3Mab-4: a Novel Anti-mouse CXCR3 Monoclonal Antibody for Flow Cytometry (preprint).

Abstract

C-X-C motif chemokine receptor 3 (CXCR3, CD183) is a G-protein-coupled receptor for CXCL9, CXCL10, and CXCL11. CXCR3 signaling induces chemotaxis of immune cells to inflammation sites and promotes inflammation in inflammatory diseases. Various mouse models to mimic the pathogenesis of each disease have been developed to understand mechanisms and evaluate therapeutics for these diseases. Although CXCR3 is an attractive target to suppress inflammation, anti-CXCR3 therapeutic agents have not been approved. In this study, we established a novel anti-mouse CXCR3 (mCXCR3) monoclonal antibody, Cx3Mab-4 (rat IgG1, kappa), using the Cell-Based Immunization and Screening method. Flow cytometric analysis demonstrated that Cx3Mab-4 bound to mCXCR3-overexpressed Chinese hamster ovary-K1 (CHO/mCXCR3) cells, but did not react to parental CHO-K1 cells. The dissociation constant of Cx3Mab-4 was determined as 1.3 × 10-9 M, indicating that Cx3Mab-4 possesses a high affinity to mCXCR3-expressing cells. Cx3Mab-4 could be useful for targeting CXCR3-expressing cells in preclinical mouse models.

Keywords

mouse CXCR3; monoclonal antibody; CBIS method

Subject

Medicine and Pharmacology, Immunology and Allergy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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