Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Cellulases and Xylanases Are Involved in the Probiotic Effect of Bacillus subtilis in Dairy Cattle Nutrition

Valeria Bontà
,
Marco Battelli
ORCID logo ,
Erlinda Rama
,
Michela Casanova
,
Lorenzo Pasotti
ORCID logo ,
Gianluca Galassi
ORCID logo ,
Stefania Colombini
ORCID logo ,
Cinzia Calvio
* ORCID logo
Version 1 : Received: 9 November 2023 / Approved: 10 November 2023 / Online: 10 November 2023 (11:31:57 CET)

How to cite: Bontà, V.; Battelli, M.; Rama, E.; Casanova, M.; Pasotti, L.; Galassi, G.; Colombini, S.; Calvio, C. Cellulases and Xylanases Are Involved in the Probiotic Effect of Bacillus subtilis in Dairy Cattle Nutrition. Preprints 2023, 2023110700. https://doi.org/10.20944/preprints202311.0700.v1 Bontà, V.; Battelli, M.; Rama, E.; Casanova, M.; Pasotti, L.; Galassi, G.; Colombini, S.; Calvio, C. Cellulases and Xylanases Are Involved in the Probiotic Effect of Bacillus subtilis in Dairy Cattle Nutrition. Preprints 2023, 2023110700. https://doi.org/10.20944/preprints202311.0700.v1

Abstract

The administration of Bacilli to dairy cows exerts a beneficial effect on dry matter intake, lactation performances and milk composition. In this work, two isogenic strains, only differing in the se-cretion level of cellulolytic and xylanolytic enzymes, were compared to establish whether B. subtilis cellulase and xylanase enzymes are involved in the positive effect exerted by these bacteria on animal nutrition. Conveniently, bacteria were grown in a newly designed growth medium based on an inexpensive waste. A multi-factorial study was conducted in which eight feed in-gredients were treated in vitro, using ruminal fluid from cannulated cows, with cultures of the two B. subtilis strains. Feed digestibility and gas production, a parameter linked to feed fermentability, were assessed. Fiber degradability was significantly higher in feeds treated with the en-zyme-overexpressing strain (10% and 5% higher than control treatment with B. subtilis-free media and the parent strain, respectively). This effect was accentuated for the most recalcitrant feeds, in which the benefits of cellulolytic and xylanolytic enzymes were maximized. Fermentability also benefited by the treatment with the enzyme-overexpressing strain, although the trend observed in gas production didn’t reach statistical difference between the two strains, and only minor changes in fermentation profiles were detected. Our results revealed that B. subtilis cellulases and xylanases effectively contribute to improving forage quality, supporting the use of Bacilli as fodder sup-plements to increase animal productivity.

Keywords

Bacillus subtilis; ruminal fermentation; forage probiotics; cellulases; xylanases

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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