Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Different Inhibition Mechanism of Atorvastatin, Olmesartan, and Resveratrol in Experimental Pathological Myofibroblast Heart Valve Model

Achmad Lefi
* ORCID logo ,
Denny Suwanto
,
Budi Baktijasa Dharmadjati
,
Yudi Her Oktaviono
,
Agus Subagjo
,
Oryza Sativa
,
Dita Aulia Rachmi
,
Ricardo Adrian Nugraha
ORCID logo
Version 1 : Received: 6 November 2023 / Approved: 9 November 2023 / Online: 9 November 2023 (07:28:49 CET)

How to cite: Lefi, A.; Suwanto, D.; Dharmadjati, B.B.; Oktaviono, Y.H.; Subagjo, A.; Sativa, O.; Rachmi, D.A.; Nugraha, R.A. Different Inhibition Mechanism of Atorvastatin, Olmesartan, and Resveratrol in Experimental Pathological Myofibroblast Heart Valve Model. Preprints 2023, 2023110578. https://doi.org/10.20944/preprints202311.0578.v1 Lefi, A.; Suwanto, D.; Dharmadjati, B.B.; Oktaviono, Y.H.; Subagjo, A.; Sativa, O.; Rachmi, D.A.; Nugraha, R.A. Different Inhibition Mechanism of Atorvastatin, Olmesartan, and Resveratrol in Experimental Pathological Myofibroblast Heart Valve Model. Preprints 2023, 2023110578. https://doi.org/10.20944/preprints202311.0578.v1

Abstract

Background. Recent studies revealed that differentiation of valvular interstitial cell into myofibroblasts played an important role in pathological valve remodeling in rheumatic valvular disease. Objective. To investigate effects of atorvastatin, olmesartan, and resveratrol on Transforming Growth Factor β1-induced fibrosis. Methods. Valvular interstitial cell was isolated from 12-weeks male New Zealand rabbit (Oryctolagus cuniculus). Culture cells was divided into 4 groups, control group, group I (0.5 mg/mL Atorvastatin), group II (100 nmol/L Olmesartan), group III (50 μM/L Resveratrol) and group IV (combinations). All group were exposed to 100 nM Transforming Growth Factor β1 for 24 hours. Results. Immunochemical staining demonstrated that cells were completely differentiated into myofibroblasts with mean expression of α-smooth muscle actin 24522.64±4566.994. Atorvastatin, olmesartan, resveratrol, and its combination significantly reduced α-smooth muscle actin expression (6823±1735.3, 6942.7±2455.9, 14176.2±3343.3, 5051.8±1612.2 respectively (p<0.001). Conclusion. Our data showed atorvastatin, olmesartan, resveratrol, and its combination significantly reduce Transforming Growth Factor β1-induced valvular fibrosis.

Keywords

Myofibroblast; Atorvastatin; Olmesartan; Resveratrol; Valvular Interstitial Cell

Subject

Medicine and Pharmacology, Cardiac and Cardiovascular Systems

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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