Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Elevated Expression of HSP72 in the Prefrontal Cortex and Hippocampus of Rats Subjected to Chronic Mild Stress and Treated with Imipramine

Adam Bielawski
ORCID logo ,
Agnieszka Zelek-Molik
ORCID logo ,
Katarzyna Rafa-Zabłocka
ORCID logo ,
Marta Kowalska
,
Piotr Gruca
,
Mariusz Papp
ORCID logo ,
Irena Nalepa
*
Version 1 : Received: 3 November 2023 / Approved: 6 November 2023 / Online: 6 November 2023 (11:35:13 CET)

A peer-reviewed article of this Preprint also exists.

Bielawski, A.; Zelek-Molik, A.; Rafa-Zabłocka, K.; Kowalska, M.; Gruca, P.; Papp, M.; Nalepa, I. Elevated Expression of HSP72 in the Prefrontal Cortex and Hippocampus of Rats Subjected to Chronic Mild Stress and Treated with Imipramine. Int. J. Mol. Sci. 2024, 25, 243. Bielawski, A.; Zelek-Molik, A.; Rafa-Zabłocka, K.; Kowalska, M.; Gruca, P.; Papp, M.; Nalepa, I. Elevated Expression of HSP72 in the Prefrontal Cortex and Hippocampus of Rats Subjected to Chronic Mild Stress and Treated with Imipramine. Int. J. Mol. Sci. 2024, 25, 243.

Abstract

The HSP70 and HSP90 family members belong to molecular chaperones that exhibit protective functions during the cellular response to stressful agents. We investigated whether the exposure of rats to chronic mild stress (CMS), a validated model of depression, affects the expression of HSP70 and HSP90 in the prefrontal cortex (PFC), hippocampus (HIP) and thalamus (Thal). Male Wistar rats were exposed to CMS for 3 or 8 weeks. The antidepressant imipramine (IMI, 10 mg/kg, i.p., daily) was introduced in the last five weeks of the long-term CMS procedure. Depressive-like behavior was verified by the sucrose consumption test. The expression of mRNA and protein was quantified by real-time PCR and Western blot, respectively. In the 8-week CMS model, stress alone elevated HSP72 and HSP90B mRNA expression in the HIP. HSP72 mRNA was increased in the PFC and HIP of rats not responding to IMI treatment vs. IMI responders. The CMS exposure increased HSP72 protein expression in the cytosolic fraction of the PFC and HIP, and this effect was diminished by IMI treatment. Our results suggest that elevated levels of HSP72 may serve as an important indicator of neuronal stress reactions accompanying depression pathology and could be a potential target for antidepressant strategy.

Keywords

chronic mild stress; depression; imipramine; heat shock protein; HSP90; inducible HSP90A; constitutive HSP90B; HSP70; inducible HSP72; constitutive HSC70

Subject

Medicine and Pharmacology, Neuroscience and Neurology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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