Molecular Identification of Fungal Species by Multiplex-qPCR in Candidal Vulvovaginitis Specimens and Antifungal Susceptibility

Inés Arrieta-Aguirre; Pilar Menéndez-Manjón; Giulia Carrano; Ander Diez; Íñigo Fernandez de Larrinoa; María-Dolores Moragues

doi:10.20944/preprints202311.0196.v1

A peer-reviewed article of this Preprint also exists.

Arrieta-Aguirre, I.; Menéndez-Manjón, P.; Carrano, G.; Diez, A.; Fernandez-de-Larrinoa, Í.; Moragues, M.-D. Molecular Identification of Fungal Species through Multiplex-qPCR to Determine Candidal Vulvovaginitis and Antifungal Susceptibility. J. Fungi 2023, 9, 1145. Arrieta-Aguirre, I.; Menéndez-Manjón, P.; Carrano, G.; Diez, A.; Fernandez-de-Larrinoa, Í.; Moragues, M.-D. Molecular Identification of Fungal Species through Multiplex-qPCR to Determine Candidal Vulvovaginitis and Antifungal Susceptibility. J. Fungi 2023, 9, 1145.

Abstract

Vulvovaginal candidiasis (VVC) is a prevalent condition affecting women worldwide. This study aimed to develop a rapid qPCR assay for accurate identification of VVC etiological agents and reduced azole susceptibility. One hundred and twenty nine vaginal samples from an outpatient clinic (Bilbao-Spain) were analyzed using culture-based methods and multiplex qPCR targeting fungal species, Candida albicans being the predominant species. Antifungal susceptibility tests revealed reduced azole susceptibility in three (3,48%) isolates. Molecular analysis identified several mutations in genes associated with azole resistance and novel mutations in TAC1 and MRR1 genes were also identified, which could contribute to drug resistance.

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Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Identification of Fungal Species by Multiplex-qPCR in Candidal Vulvovaginitis Specimens and Antifungal Susceptibility

Abstract

Keywords

Subject

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