Version 1
: Received: 30 October 2023 / Approved: 30 October 2023 / Online: 31 October 2023 (05:18:01 CET)
Version 2
: Received: 31 October 2023 / Approved: 31 October 2023 / Online: 31 October 2023 (09:01:09 CET)
How to cite:
Pan, Z.; Xie, C.; Yan, X.T.; Su, Y.M.; Haq, A.U.; Wang, J. Characteristic Volatile Compounds of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen and Their Potential Anti-oxidant Activities. Preprints2023, 2023101959. https://doi.org/10.20944/preprints202310.1959.v2
Pan, Z.; Xie, C.; Yan, X.T.; Su, Y.M.; Haq, A.U.; Wang, J. Characteristic Volatile Compounds of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen and Their Potential Anti-oxidant Activities. Preprints 2023, 2023101959. https://doi.org/10.20944/preprints202310.1959.v2
Pan, Z.; Xie, C.; Yan, X.T.; Su, Y.M.; Haq, A.U.; Wang, J. Characteristic Volatile Compounds of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen and Their Potential Anti-oxidant Activities. Preprints2023, 2023101959. https://doi.org/10.20944/preprints202310.1959.v2
APA Style
Pan, Z., Xie, C., Yan, X.T., Su, Y.M., Haq, A.U., & Wang, J. (2023). Characteristic Volatile Compounds of <em>Phlomoides rotata</em> (Benth. ex Hook. f.) Mathiesen and Their Potential Anti-oxidant Activities. Preprints. https://doi.org/10.20944/preprints202310.1959.v2
Chicago/Turabian Style
Pan, Z., Anwar Ul Haq and Jian Wang. 2023 "Characteristic Volatile Compounds of <em>Phlomoides rotata</em> (Benth. ex Hook. f.) Mathiesen and Their Potential Anti-oxidant Activities" Preprints. https://doi.org/10.20944/preprints202310.1959.v2
Abstract
Due to the low content, few studies are focused on the volatile compounds of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen (PR, syn. Lamiophlomis rotata (Benth.) Kudô). However, such volatile compounds may have important pharmacological activities such as antioxidant, anticancer. In order to identify the chemical markers (CMs) in the essential oils (Es) and explore the potential bioactivities, we first conduct a thoroughly investigation on the volatile compounds and evaluate their anti-oxidant activities (AOAs), to the best of our knowledge. Light yellow Es with a fresh and elegant smell are obtained by hydro-distillation with an average yield of 0.11% (mL/g). After separation through cryoprecipitation, the crystals (Cs) and EOs removed crystals (RCs) were gotten, respectively. A total of 81 components are qualified and quantified in the Es, Cs and RCs, in which 44 ones are first reported from PR. As for content, the main compounds are long-chain fatty acids (LCFAs) and their esters. Among them, palmitic acid (PA), myristic acid (MA), linoleic acid (LA), oleic acid (OA) and methyl palmitate are highlighted, Besides, hexahydrofarnesyl acetone and phytol are also prominent Four kinds of C13-norisoprenoids are first reported from PR, in which, trans-β-damascenone is potent in flavour. In sum, these eight representative compounds can be chosen as the CMs. The AOAs of Es, Cs, RCs and four CMs such as PA, MA, LA and OA are evaluated by in vitro assays. In general, PA and MA present pro-oxidant activities (POAs) or weak AOAs. LA and OA show POAs or weak AOAs in lower concentration group, whereas demonstrate medium or strong AOAs in higher concentration group. Usually, the RCs demonstrate stronger AOAs and the Cs present weaker AOAs compared with those of the corresponding Es, which should be related to their different content of PA. This study can give some hints on the utilization of such Es which are abundant in LCFAs.
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received:
31 October 2023
Commenter:
Zheng Pan
Commenter's Conflict of Interests:
Author
Comment:
This paper is a modification of the “molecules-2570729” which was submitted to Special issue "Essential Oils: From Extraction to Application" previously. After full consideration of the reviewers’ comment, we have revised it a lot and decided to sent it to the Special Issue “New Insights into Bioactive Compounds from Natural Sources”.The formerly submitted paper and revised paper was concurrently submitted to https://www.preprints.org/ such as https://www.preprints.org/manuscript/202307.0092/v1,https://www.preprints.org/manuscript/202307.0092/v2,https://www.preprints.org/manuscript/202307.0092/v3,https://www.preprints.org/manuscript/202307.0092/v4. It can be seen from version 1 (v1) to version 4 (v4), the paper was improved step by step after adopting the reviewers’ comment.
Commenter: Zheng Pan
Commenter's Conflict of Interests: Author