Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Au-Coated ZnO Surface-Enhanced Raman Scattering (SERS) Substrates: Synthesis, Characterization, and Applications in Exosome Detection

Samuel Adesoye
ORCID logo ,
Saqer Al Abdullah
,
Anjali Kumari
,
Gayani Pathiraja
ORCID logo ,
Kyle Nowlin
ORCID logo ,
Kristen Dellinger
* ORCID logo
Version 1 : Received: 24 October 2023 / Approved: 25 October 2023 / Online: 25 October 2023 (09:59:58 CEST)

A peer-reviewed article of this Preprint also exists.

Adesoye, S.; Abdullah, S.A.; Kumari, A.; Pathiraja, G.; Nowlin, K.; Dellinger, K. Au-Coated ZnO Surface-Enhanced Raman Scattering (SERS) Substrates: Synthesis, Characterization, and Applications in Exosome Detection. Chemosensors 2023, 11, 554. Adesoye, S.; Abdullah, S.A.; Kumari, A.; Pathiraja, G.; Nowlin, K.; Dellinger, K. Au-Coated ZnO Surface-Enhanced Raman Scattering (SERS) Substrates: Synthesis, Characterization, and Applications in Exosome Detection. Chemosensors 2023, 11, 554.

Abstract

Developing a biomolecular detection method that minimizes photodamage while maintaining an environment suitable for biological constituents to maintain their physiological state is expected to drive new diagnostic and mechanistic breakthroughs. In addition, ultra-sensitive diagnostic platforms are needed for rapid and point-of-care technologies for various diseases. Considering this, surface-enhanced Raman scattering (SERS) is proposed as a non-destructive and sensitive approach to address the limitations of fluorescence, electrochemical, and other optical detection techniques. However, to advance the applications of SERS, novel approaches to enhance the signal of substrate materials are needed to improve reproducibility and costs associated with manu-facture and scale-up. Due to their physical properties and synthesis, semiconductor-based nanostructures have gained increasing recognition as SERS substrates; however, low signal en-hancements have offset their widespread adoption. To address this limitation and assess the po-tential for use in biological applications, zinc oxide (ZnO) was coated with different concentra-tions (0.01-0.1 M) of gold (Au) precursor. When crystal violet (CV) was used as a model target with the synthesized substrates, the highest enhancement was obtained with ZnO coated with 0.05 M Au precursor. This substrate was subsequently applied to differentiate exosomes derived from three cell types to provide insight into their molecular diversity. We anticipate this work will serve as a platform for colloidal hybrid SERS substrates in future bio-sensing applications.

Keywords

Raman spectroscopy; biosensing; exosomes; hybrid nanoparticles; zinc oxide

Subject

Chemistry and Materials Science, Nanotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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