Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Bioimaging of Genomic DNA with Nitrogen-Doped Carbon Quantum Dots Produced from Raphanus. sativus

Version 1 : Received: 13 October 2023 / Approved: 16 October 2023 / Online: 16 October 2023 (11:03:15 CEST)

How to cite: Sener, B.; Ates, I. Bioimaging of Genomic DNA with Nitrogen-Doped Carbon Quantum Dots Produced from Raphanus. sativus . Preprints 2023, 2023100941. https://doi.org/10.20944/preprints202310.0941.v1 Sener, B.; Ates, I. Bioimaging of Genomic DNA with Nitrogen-Doped Carbon Quantum Dots Produced from Raphanus. sativus . Preprints 2023, 2023100941. https://doi.org/10.20944/preprints202310.0941.v1

Abstract

In recent years, genetic research has been developing rapidly and taking on a larger role in research and medicine. The synthesis of any particle that can be used in this research can pave the way for new developments in this field. This research aims to develop a chemical method to produce nitrogen-doped carbon quantum dots - economical and bio-sourced substances that bind to genomic DNA. Carbon quantum dots are produced by the green synthesis hydrothermal method from radish, which is abundant in nature, and economically affordable. Nitrogen is added to increase the fluorescence properties of the produced carbon quantum dots and to improve their binding to DNA. The synthesized nitrogen-doped carbon quantum dots (N-CQDs) were tested through chromosome imaging subsequently. It was shown that N-CQDs can bind to the chromosomes, and N-CQDs reflected green light better than DAPI under the FITC filter, a potential advantage of using N-CQDs over DAPI in chromosome imaging.

Keywords

carbon quantum dots; fluorescent properties; imaging; biotechnology; intracellular bioimaging; quantum dots; nitrogen-doped carbon quantum dots; DAPI; FITC

Subject

Biology and Life Sciences, Biology and Biotechnology

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