Performance of a Nanoribbon Biosensor Sensitized with Aptamers and Antibodies upon Detection of HCVcoreAg

Yuri Ivanov; Kristina Malsagova; Kristina Goldaeva; Tatyana Pleshakova; Andrey Kozlov; Rafael Galiullin; Ivan Shumov; Vladimir Popov; Irina Abramova; Ekaterina Nevedrova; Angelina Vinogradova; Vadim Ziborov; Oleg Petrov; Alexander Dolgoborodov; Alexander Archakov

doi:10.20944/preprints202310.0841.v1

How to cite: Ivanov, Y.; Malsagova, K.; Goldaeva, K.; Pleshakova, T.; Kozlov, A.; Galiullin, R.; Shumov, I.; Popov, V.; Abramova, I.; Nevedrova, E.; Vinogradova, A.; Ziborov, V.; Petrov, O.; Dolgoborodov, A.; Archakov, A. Performance of a Nanoribbon Biosensor Sensitized with Aptamers and Antibodies upon Detection of HCVcoreAg . Preprints 2023, 2023100841. https://doi.org/10.20944/preprints202310.0841.v1 Ivanov, Y.; Malsagova, K.; Goldaeva, K.; Pleshakova, T.; Kozlov, A.; Galiullin, R.; Shumov, I.; Popov, V.; Abramova, I.; Nevedrova, E.; Vinogradova, A.; Ziborov, V.; Petrov, O.; Dolgoborodov, A.; Archakov, A. Performance of a Nanoribbon Biosensor Sensitized with Aptamers and Antibodies upon Detection of HCVcoreAg . Preprints 2023, 2023100841. https://doi.org/10.20944/preprints202310.0841.v1

Abstract

The performance of the nanoribbon biosensor upon the use of two different types of molecular probes — the antibodies and the aptamers against HCVcoreAg — has been tested. The sensor chips employed are based on “silicon-on-insulator structures”. Two different HCVcoreAg preparations have been tested: recombinant b-galactosidase-conjugated HCVcoreAg (“Virogen”, USA) and recombinant HCVcoreAg (“Vector-Best”, Russia). Upon the detection of either type of the antigen preparation, the lowest concentration of the antigen detectable in buffer with pH 5.1 has been found to be approximately equal, amounting to ~10^–14 M. This value has been found to be similar upon the use of either type of molecular probes.

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Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Performance of a Nanoribbon Biosensor Sensitized with Aptamers and Antibodies upon Detection of HCVcoreAg

Abstract

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