Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Molecular Evaluation of FLC Homologs and Coordinating Regulators on the Flowering Responses to Chilling Temperature in Cabbage (Brassica oleraceae var. capitata) Genotypes

Ju-Young Ahn
ORCID logo ,
Saminathan Subburaj
ORCID logo ,
Fanzhuang Yan
,
Yao Jian
,
Chandrasekaran Ajithan
,
Kyoung-Gu Ahn
,
Geung-Joo Lee
* ORCID logo
Version 1 : Received: 12 October 2023 / Approved: 12 October 2023 / Online: 12 October 2023 (09:40:23 CEST)

How to cite: Ahn, J.; Subburaj, S.; Yan, F.; Jian, Y.; Ajithan, C.; Ahn, K.; Lee, G. Molecular Evaluation of FLC Homologs and Coordinating Regulators on the Flowering Responses to Chilling Temperature in Cabbage (Brassica oleraceae var. capitata) Genotypes. Preprints 2023, 2023100793. https://doi.org/10.20944/preprints202310.0793.v1 Ahn, J.; Subburaj, S.; Yan, F.; Jian, Y.; Ajithan, C.; Ahn, K.; Lee, G. Molecular Evaluation of FLC Homologs and Coordinating Regulators on the Flowering Responses to Chilling Temperature in Cabbage (Brassica oleraceae var. capitata) Genotypes. Preprints 2023, 2023100793. https://doi.org/10.20944/preprints202310.0793.v1

Abstract

Cabbage (B. oleracea var. capitata) contains three FLC homolog genes (BoFLC1, BoFLC2, and BoFLC3) and one pseudogene (BoFLC5) that responds to low temperatures necessary for flowering. We isolated three BoFLC-encoding genes from early-, mid-, and late-flowering cabbage plants. Leaf samples were collected for RNA extraction and expression analysis. Gene structure analysis and phylogenetic comparison were performed for intra- or interspecific relationships of the BoFLC homologs. Gene expression related to flowering regulators (BoGI, BoCOOLAIR, and BoVIN3) was analyzed. While BoFLC genes commonly have seven exons and six introns of 3,361–4.384 bp, variations in insertion or deletion were evident between the early- and late-flowering genotypes. Repressed expression of BoFLC 1, 2, and 3 genes under chilling temperatures appeared to occur from 8 weeks after vernalization. Higher expression levels of GI, COOLAIR, and VIN3 were initiated 7 weeks after chilling (WAT) treatment in the early flowering genotype. The vernalization trigger repressed the expression of BoFLC homologs. This study provides molecular insights into BoFLC homologs between early- and late-flowering cabbage genotypes, in which structural variations in BoFLC1 appeared to be important as a binding motif to flowering regulating factors such as BoGI, BoCOOLAIR, and BoVIN3 for which motif analysis is further implemented.

Keywords

BoFLC homologs; cabbage flowering; flowering regulator; structural variation; vernalization

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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