Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Impact of Perinatal Co-exposure to Chlorpyrifos and a High-Fat Diet on Kisspeptin and GnRHR Presence and Reproductive Organs

Marwa Lahimer
,
Narimane Djekkoun
,
Sophian Tricotteaux-Zarqaoui
,
Aurélie Corona
ORCID logo ,
Isabelle Lafosse
,
Habib Ben Ali
,
Mounir Ajina
,
Véronique Bach
,
Moncef BenKhalifa
,
Hafida Khorsi-Cauet
*
Version 1 : Received: 24 August 2023 / Approved: 24 August 2023 / Online: 25 August 2023 (08:20:07 CEST)

A peer-reviewed article of this Preprint also exists.

Lahimer, M.; Djekkoun, N.; Tricotteaux-Zarqaoui, S.; Corona, A.; Lafosse, I.; Ali, H.B.; Ajina, M.; Bach, V.; Benkhalifa, M.; Khorsi-Cauet, H. Impact of Perinatal Coexposure to Chlorpyrifos and a High-Fat Diet on Kisspeptin and GnRHR Presence and Reproductive Organs. Toxics 2023, 11, 789. Lahimer, M.; Djekkoun, N.; Tricotteaux-Zarqaoui, S.; Corona, A.; Lafosse, I.; Ali, H.B.; Ajina, M.; Bach, V.; Benkhalifa, M.; Khorsi-Cauet, H. Impact of Perinatal Coexposure to Chlorpyrifos and a High-Fat Diet on Kisspeptin and GnRHR Presence and Reproductive Organs. Toxics 2023, 11, 789.

Abstract

Emerging evidence has indicated the involvement of extra-hypothalamic kisspeptin and GnRHR in reproductive function. In this study, we evaluate if maternal exposure to the pesticide chlorpyrifos (CPF) and/or a high-fat diet (HFD) have an impact on the expression of kisspeptin and GnRHR in reproductive organ of offspring’s rats. 16 Pregnant rats are divided into 4 groups, control group (n=4), CPF group (4 rats exposed daily to 1/mg/kg/day), HFD group (4 rats randomly fed a 5,25 kcal/g HFD), co-exposed group (4 rats exposed to CPF and HDF). At postnatal development PND60, males and female offspring were sacrificed. The reproductive organs (ovary and testis) were removed and histological, immunohistological analysis and in silico quantification (software Tissuefax, HistoQuest) was assessed to investigate the impact of different treatment on kisspeptin and GnRHR expression in reproductive organs. The main outcomes of the study showed a significant decrease in rat offspring’s body weight in CPF group from PND30 andPND60 (p<0.05 and p<0.01, respectively). Histological analysis shows a significant increase in atretic follicle and abnormal testis structure with germ cell desquamation at CPF exposed group. The immunodetection quantification of protein shows a significant decrease in GnRHR and kisspeptin in HFD and CPF exposed group, respectively in testis rat offspring. Perinatal exposure to CPF and HFD exposure affect the reproduction function of rat offspring.

Keywords

chlorpyrifos; high-fat diet; kisspeptin; GnRHR; ovary; testis; rat

Subject

Public Health and Healthcare, Public, Environmental and Occupational Health

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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