Version 1
: Received: 9 August 2023 / Approved: 10 August 2023 / Online: 10 August 2023 (13:21:14 CEST)
How to cite:
Jiang, X.; Fan, X. A Mass Mortality Event Caused by Pathogenic Escherichia coli in a Donkey Farm. Preprints2023, 2023080869. https://doi.org/10.20944/preprints202308.0869.v1
Jiang, X.; Fan, X. A Mass Mortality Event Caused by Pathogenic Escherichia coli in a Donkey Farm. Preprints 2023, 2023080869. https://doi.org/10.20944/preprints202308.0869.v1
Jiang, X.; Fan, X. A Mass Mortality Event Caused by Pathogenic Escherichia coli in a Donkey Farm. Preprints2023, 2023080869. https://doi.org/10.20944/preprints202308.0869.v1
APA Style
Jiang, X., & Fan, X. (2023). A Mass Mortality Event Caused by Pathogenic Escherichia coli in a Donkey Farm. Preprints. https://doi.org/10.20944/preprints202308.0869.v1
Chicago/Turabian Style
Jiang, X. and Xuewei Fan. 2023 "A Mass Mortality Event Caused by Pathogenic Escherichia coli in a Donkey Farm" Preprints. https://doi.org/10.20944/preprints202308.0869.v1
Abstract
Escherichia coli (E. coli) is a common pathogen in veterinary clinical infections, typically not causing disease. However, under certain conditions, it can cause severe diarrhea, septicemia, and respiratory infections in domestic animals. Here, we report a large-scale mortality event in young donkeys, caused by E. coli infection in a farm with a herd of 340 donkeys, of which over 100 were under 4 months old. The characteristics of the E. coli strain were determined through clinical observation, complete blood count, blood biochemical analysis, H&E staining, histopathological examination, 16S rRNA analysis, and antibiotic sensitivity testing. A mice experiment using the isolated bacteria was also conducted, resulting in the onset of clinical and pathological symptoms similar to those observed in the donkeys. Molecular Evolutionary Genetic Analysis (MEGA) showed that the isolated bacteria, named CEG-GZL20, shared 99.98% sequence identity with E. coli. CEG-GZL20 exhibited high antibiotic resistance and only showed sensitivity to two antibiotics, Tetracycline and Gentamicin. As E. coli is a key component of the gut microbiota, responsible for digestion, nutrient absorption, and immune health, diseases and mortality caused by E. coli have significant implications for animal husbandry and human health.
Keywords
donkey; Escherichia coli; antibiotic resistant
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
