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Effect of Bioactive Peptides From Atlantic Salmon Processing By-Products on Oxyntopeptic and Enteroendocrine Cells of the Gastric Mucosa of European Seabass and Gilthead Seabream
Clavenzani, P.; Lattanzio, G.; Bonaldo, A.; Parma, L.; Busti, S.; Oterhals, Å.; Romarheim, O.H.; Aspevik, T.; Gatta, P.P.; Mazzoni, M. Effects of Bioactive Peptides from Atlantic Salmon Processing By-Products on Oxyntopeptic and Enteroendocrine Cells of the Gastric Mucosa of European Seabass and Gilthead Seabream. Animals2023, 13, 3020.
Clavenzani, P.; Lattanzio, G.; Bonaldo, A.; Parma, L.; Busti, S.; Oterhals, Å.; Romarheim, O.H.; Aspevik, T.; Gatta, P.P.; Mazzoni, M. Effects of Bioactive Peptides from Atlantic Salmon Processing By-Products on Oxyntopeptic and Enteroendocrine Cells of the Gastric Mucosa of European Seabass and Gilthead Seabream. Animals 2023, 13, 3020.
Clavenzani, P.; Lattanzio, G.; Bonaldo, A.; Parma, L.; Busti, S.; Oterhals, Å.; Romarheim, O.H.; Aspevik, T.; Gatta, P.P.; Mazzoni, M. Effects of Bioactive Peptides from Atlantic Salmon Processing By-Products on Oxyntopeptic and Enteroendocrine Cells of the Gastric Mucosa of European Seabass and Gilthead Seabream. Animals2023, 13, 3020.
Clavenzani, P.; Lattanzio, G.; Bonaldo, A.; Parma, L.; Busti, S.; Oterhals, Å.; Romarheim, O.H.; Aspevik, T.; Gatta, P.P.; Mazzoni, M. Effects of Bioactive Peptides from Atlantic Salmon Processing By-Products on Oxyntopeptic and Enteroendocrine Cells of the Gastric Mucosa of European Seabass and Gilthead Seabream. Animals 2023, 13, 3020.
Abstract
The aim of the present study was to evaluate the effects on the presence and distribution of oxyntopeptic cells (OPs) and enteroendocrine cells (EECs) expressing GHR, NPY and SOM in the gastric mucosa of European seabass and seabream of dietary levels of bioactive peptides (BPs) obtained from salmon processing by-products (in silico approach). In this study, 27 seabass and 27 seabreams were divided into three experimental groups: a control group (CTR) fed a control diet and two groups fed different levels of BP to replace fishmeal: 5% BP (BP5%) and 10% BP (BP10%). The stomach of each fish was sampled and processed for immunohistochemistry. Some SOM, NPY and GHR-IR cells exhibited alternating “open type” and “closed type” EECs morphologies. In seabream gastric mucosa, the BP10% group (16.8 ± 7.5) showed a significant change in NPY-IR cell number compared to CTR (CTR 8.5 ± 4.8) and BP5% (BP10% vs. CTR P ≤ 0.01; BP10% vs. BP5% P ≤ 0.05). In seabream, SOM-IR cells in the BP 10% diet (16.8 ± 3.5) differed from those in CTR (12.5 ± 5) (CTR vs. BP 10% P ≤ 0.05) and BP 5% (12.9 ± 2.5) (BP 5% vs. BP 10% P ≤ 0.01). In seabass, EEC SOM-IR cells increased at 10 % BP (5.3 ± 0.7) compared to 5 % BP (4.4 ± 0.8) (5 % BP vs. 10 % BP P ≤ 0.05). The results obtained suggest that salmon BPs have a promising impact as circulating ingredients for seabass and seabream.
Keywords
gilthead seabream and European seabass; bioactive peptide; oxyntopeptic cells; neuropeptide Y; somatostatin and ghrelin
Subject
Biology and Life Sciences, Aquatic Science
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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