Palumbo, C.; Sisi, F.; Checchi, M. CAM Model: Intriguing Natural Bioreactor for Sustainable Research and Reliable/Versatile Testing. Biology2023, 12, 1219.
Palumbo, C.; Sisi, F.; Checchi, M. CAM Model: Intriguing Natural Bioreactor for Sustainable Research and Reliable/Versatile Testing. Biology 2023, 12, 1219.
Palumbo, C.; Sisi, F.; Checchi, M. CAM Model: Intriguing Natural Bioreactor for Sustainable Research and Reliable/Versatile Testing. Biology2023, 12, 1219.
Palumbo, C.; Sisi, F.; Checchi, M. CAM Model: Intriguing Natural Bioreactor for Sustainable Research and Reliable/Versatile Testing. Biology 2023, 12, 1219.
Abstract
We are witnessing the revival of the CAM model, which was already used in the past by some authors studying angiogenesis and anti-cancer drugs, that now offers a refined model to fill, in the translational meaning, the gap between in vitro-in vivo studies. It can be used for a wide range of purposes, from testing cytotoxicity, pharmacokinetics, tumorigenesis, invasion, to action mechanisms of molecules, and validation of new materials from tissue engineering research. CAM model is easy to use with a fast outcome and makes experimental research more sustainable since allowing to replace, reduce and refine pre-clinical experimentation ("3Rs" rules [1]). This re-view aims to highlight some unique potentials that CAM-assay presents; in particular, the authors intend to use in the next future the CAM model to verify, in a microenvironment comparable to in vivo conditions, albeit simplified, the angiogenic ability of functionalized 3D constructs to be used in regenerative medicine strategies in the recovery of skeletal injuries of critical size (CSD) that do not repair spontaneously. For this purpose, organotypic cultures will be planned on sever-al CAMs set up in temporal sequence: a sort of organ model for assessing CSD conducted in the CAM bioreactor, rather than in vivo.
Keywords
chorioallantoic Membrane (CAM); angiogenesis; organotypic culture; engineered 3D scaffold
Subject
Biology and Life Sciences, Anatomy and Physiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
