Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Reduced Endothelial Nitric Oxide Involvement in Duck Basilar Arterial Relaxation

Siyuan Wu
,
Tomoki Ootawa
ORCID logo ,
Henry Smith
,
Ryoya Sekio
,
Md. Zahorul Islam
ORCID logo ,
Ha Thi Thanh Nguyen
ORCID logo ,
Yasuhiro Uno
,
Mitsuya Shiraishi
,
Atsushi Miyamoto
* ORCID logo
Version 1 : Received: 2 August 2023 / Approved: 3 August 2023 / Online: 3 August 2023 (14:50:39 CEST)

A peer-reviewed article of this Preprint also exists.

Wu, S.; Ootawa, T.; Sekio, R.; Smith, H.; Islam, M.Z.; Nguyen, H.T.T.; Uno, Y.; Shiraishi, M.; Miyamoto, A. Reduced Nitric Oxide Synthase Involvement in Aigamo Duck Basilar Arterial Relaxation. Animals 2023, 13, 2740. Wu, S.; Ootawa, T.; Sekio, R.; Smith, H.; Islam, M.Z.; Nguyen, H.T.T.; Uno, Y.; Shiraishi, M.; Miyamoto, A. Reduced Nitric Oxide Synthase Involvement in Aigamo Duck Basilar Arterial Relaxation. Animals 2023, 13, 2740.

Abstract

The basilar arterial endothelium mediates blood-vessel relaxation, partly through release of nitric oxide (NO). Apoptosis of cerebrovascular endothelial cells is linked to high mortality rate in chickens infected with highly pathogenic avian influenza virus, but interestingly ducks exhibit greater resistance to this virus. In this study, we examined the responsiveness of duck basilar arteries (BAs) to various vasoactive substances, including 5-hydroxytryptamine (5-HT), histamine (His), angiotensin (Ang) II, noradrenaline (NA), acetylcholine (ACh), and avian bradykinin ornithokinin (OK), aiming to characterize the receptor subtypes involved and the role of endothelial NO in vitro. Our findings suggest that arterial contraction is mediated by 5-HT1 and H1 receptors, while relaxation is induced by β3-adrenergic and M3 receptors. Additionally, OK elicited a biphasic response in duck BAs, and Ang II had no effect. Endothelial NO appears to be crucial in relaxation mediated by M3 and OK receptors, but not β3-adrenergic receptors, in the duck BA. The reduced endothelial NO involvement in the receptor-mediated relaxation response in duck BAs represents a clear difference from the corresponding response reported in chicken BAs. This physiological difference may explain differences in lethality between ducks and chickens when vascular endothelial cells are infected by the virus.

Keywords

cerebral artery; duck; endothelium; highly pathogenic avian influenza; nitric oxide

Subject

Medicine and Pharmacology, Veterinary Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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