Synthesis of Aminobisphosphinates through a Cascade Reaction between Hypophosphorous Acid and Bis(trimethylsilyl)imidates Mediated by ZnI₂

1. Introduction

The synthesis of phosphorylated molecules still represents a major challenge for organic chemists to propose new drugs.[1,2,3,4] In the midst of them, phosphinate derivatives (R²R³PO₂R¹) have gained attention in medicinal chemistry for their potential as bioactive compounds and drug candidates thanks to their ability to mimic phosphate or carboxylate function. Indeed, the presence of P-C bond imparts chemical stability towards hydrolysis, whether it occurs through chemical or enzymatic processes.[2,4]

Hence, the development of efficient methodologies is crucial to access phosphorylated scaffolds. The formation of the P-C bond can be managed by several pathways such as transition metal catalysis, radical reactions, nucleophilic additions or substitutions.[5] Among these methods, the use of silylated phosphonite II represents a versatile tool operating in a smooth way and thus compatible with functionalized molecules. Moreover, they are easily accessible by reaction between H-phosphinate I and a silylated agent like HMDS, TMSCl or bis(trimethylsilyl)acetamide (BSA) depicted in Figure 1. The sila-Arbuzov reaction of silylated phosphonites II on alkyl halides as electrophiles can provide substituted alkyl phosphinates. Aldehydes, ketones and imines can also undergo the nucleophilic attack of silylated phosphonites via Abramov reaction to give various α-hydroxy- and α-aminophosphinates respectively. In addition, the Michael addition on α,β-unsaturated ketones can selectively take place to form functionalized substituted phosphinates.[6]

Our group have contributed to the use of the simplest silylated phosphonite: bis(trimethylsilyl)phosphonite II (R¹= TMS, R²= H: BTSP) obtained starting from hypophosphorous acid (H₃PO₂) and BSA as silylating agent. First, we have demonstrated that only 2 equivalents of BSA were required to fully transform H₃PO₂ into BTSP despite large excesses of silylated agents were previously employed in the literature.[6] Then, the subsequent addition on aldehydes and ketones provided various α-hydroxyphosphinates as sodium salts in good to excellent yields.[7]

Moreover, we have also performed the successive double nucleophilic addition of BTSP onto trivalent electrophiles as acyl chlorides which enabled the formation of hydroxymethylenebisphosphinates (HMBPi) via silylated α-ketophosphinates in good to excellent yields and short reaction times.[8,9] Thereafter, this easily handled methodology was successfully used on other trivalent electrophiles, like anhydrides and activated esters, which led to more functionalized HMBPi derivatives in good yields.[10]

This method has been subsequently transposed to synthesize hydroxymethylene(phosphinyl)phosphonate derivatives (HMPPs) which have consisted of adding BTSP on in situ pre-formed α-ketophosphonates starting from trimethylphosphite and acyl chlorides. This one-pot procedure allowed the preparation of original HMPPs in which no purification of intermediate species was required (Figure 1, (a)).[11]

Besides, these methodologies were applied to the synthesis of aminoalkyl-substituted HMBPi and HMPPs which are analogues of hydroxymethylenebisphosphonates (HMBPs) currently used in clinics to treat bone diseases such as osteoporosis, solid tumor metastases or myeloma bone disease.[12,13,14,15,16,17,18,19] Moreover, HMBPs have shown interesting antitumor properties on in vitro and in vivo models of soft tissue primary tumor. As a result, the antiproliferative activities of these newly synthesized HMBPi and HMPPs have been evaluated on various cancer cell lines and encouraging results were obtained especially on A549 cells (Figure 1, (b)).[11]

Additionally, several α-aminomethylenebisphosphonates (AMBPs) exhibit biological activities which include antiparasitic,[20,21] antibacterial,[22] herbicidal [23,24] and bone resorption inhibitor [14,25] (Figure 2, (a)). The access to AMBPs is well documented in the literature. [26] Indeed, several approaches display, the double phosphonylation of amides and nitriles mediated by various Lewis acids,[27,28,29,30] a three-component reaction of amines with orthoformate and phosphites,[31,32,33,34,35,36,37,38,39,40] and a Beckmann transposition of oximes in the presence of phosphites [41,42] (Figure 2, (b)). Alternatively, only limited examples were reported for the synthesis of aminomethylenebisphosphinates (AMBPi) and their biological activities remain unknown to date.[43,44] Here, the strategy usually consists of adding in situ pre-formed BTSP onto ethyl formimidate hydrochloride or onto substituted amides in the presence of TMSOTf, respectively (Figure 2, (c)). In this case, only few N-substituted aminomethylenebisphosphinates (AMBPi) were synthesized.

As a result of these works, our team has decided to pursue exploring the nucleophilic potential of BTSP towards less reactive trivalent electrophiles such as nitriles.

In this case, aminomethylenebisphosphinate (AMBPi) scaffolds will be formed through the successive double addition of BTSP on nitriles. However, the lack of nitrile reactivity should require the use of a Lewis acid as was demonstrated in AMBP series.

Our initial experiment consisted of the in situ formation of BTSP by silylation of H₃PO₂ in the presence of BSA in THF followed by the addition of benzonitrile and ZnCl₂ as Lewis acid (Figure 3, (a)). Finally, the reaction mixture was stirred under reflux as no conversion was observed at room temperature. The reaction evolution was monitored by ³¹P and ³¹P{¹H} NMR experiments. After refluxing 6 h, the complete conversion of BTSP was observed and the following methanolysis conducted to an AMBPi derivative. However, the careful analysis of the ¹H, ¹³C spectra and mass spectroscopy indicates the formation of an α-aminomethylenebisphosphinate including a methyl substituent instead of the expected phenyl group. Consequently, the reaction did not occur on nitrile but on the N-silylacetamide generated during the silylation step in the presence of bis(trimethylsilyl)acetamide which is in accordance with some reported works previously mentioned.[30] N-Silylacetamide appears to be a better electrophile than benzonitrile towards the attack of the nucleophilic BTSP.

In view of this unexpected result, we decided to explore the feasibility of developing a cascade reaction in which a bis(trimethylsilyl)imidate 1 could silylate H₃PO₂ to simultaneously generate nucleophilic BTSP and an electrophilic N-silylamide 2. (Figure 3, (b)). Subsequently and in the presence of a Lewis acid, these products could react together to enable the formation of AMBPi derivatives. Herein, we present our endeavors to develop an efficient cascade process promoted by a Lewis acid to furnish α-aminomethylenebisphosphinates 3 (AMBPi).

2. Results & discussion

2.1. Synthesis of bis(silyl)imidates 1

First, we focused on the synthesis of N,O- bis(trimethylsilyl)imidates 1 [45,46] which could be achieved by silylation of amides [47,48] or by adding LiHMDS on acyl chlorides [45,49,50,51,52] (Scheme 1).

In both cases, the reaction allows to form the N,N-bis(silyl)amides 4 which instantly tautomerizes to the more stable N,O-bis(trimethylsilyl)imidates 1.[45,51]. Indeed, the reaction monitoring by ¹³C NMR enabled to only detect the quaternary carbon of 1 at ~160-165 ppm. The signal at ~147 ppm corresponding to N,N-bis(silyl)amides 4 was only observed when 2.2 equivalents of TMSOTf/Et₃N were sequentially added in two portions to the corresponding acetamide. In our study, the reaction between amides and TMSOTf/Et₃N was selected due to its ease of implementation and higher efficiency.

2.2. Optimization of the reaction between N,O-bis(trimethylsilyl)imidates and phosphorous acid mediated by Lewis acid

The reaction was firstly carried out between hypophosphorous acid and commercially available N,O-bis(trimethylsilyl)acetamide 1a (R= Me) (Table 1). The silylation was monitored by ³¹P NMR and was completed after 40 minutes at 0 °C.

Thereafter, various Lewis acids were screened for the second reaction between trimethylsilylacetamide 2a and BTSP (Table 1, entries 1-4). In the presence of zinc halides, AMBPi 3a was similarly obtained in good conversions and isolated yields after purification (Table 1, entries 1, 2). However, it was noted that the reaction rate is higher with ZnI₂ than with ZnCl₂, as the former allowed to complete the reaction after only 1.5 hours, whereas the latter took 18 hours. When TMSOTf was used as Lewis acid, the reaction was able to proceed at 0 °C after only 30 minutes and furnished AMBPi 3a in 75 % yield (Table 1, entry 3). In contrast, no conversion was observed in the presence of BF₃.OEt₂ regardless of the temperature and reaction time (Table 1, entry 4).

Then, the same reactions were performed with freshly prepared N,O-bis(trimethylsilyl)acetamide 1a (R= Me) in the presence of ZnX₂ or TMSOTf (Table 1 : entries 5-7 versus 1-3). In these cases, the reactions provided the same results independently of the Lewis acids as expected.

To explore the potential range of the reaction, additional N,O-bis(trimethylsilyl)imidates 1b (R= Pr) and 1c (R= Ph) were initially combined with H₃PO₂, and the resulting blend was subsequently subjected to various Lewis acids (Table 1, entries 8-13).

Concerning the use of N,O-bis(trimethylsilyl)butanimidate 1b, the silylation of H₃PO₂ was completed after 40 minutes at 0 °C. The consequently double addition of BTSP on the corresponding silylamide 2b successfully ensued in the presence of ZnX₂ to furnish the expected AMBPi 3b after methanolysis and purification (Table 1, entries 8,9). As previously observed, the reaction rate is higher for ZnI₂ than for ZnCl₂. However, we noted a dramatic drop of the conversion into 2b in the presence of TMSOTf, as a major disproportionation of BTSP was observed (Table 1, entry 10).

Upon investigating the reactivity of aromatic bis(trimethylsilyl)amide (Table 1, entries 11-13), it was revealed that among the various Lewis acids tested, zinc iodide uniquely mediated the attack of BTSP onto 2c, leading to the proper formation of AMBPi 3c (Table 1, entry 12). Indeed, no reaction took place in the presence of TMSOTf (Table 1, entry 13); moreover, the use of zinc chloride resulted in the formation of α-aminophosphinate 5c and a major disproportionation of BTSP into silylated phosphorus derivatives (Table 1, entry 11). Furthermore, the reactivity of zinc chloride seems inadequate to promote the sila-Arbuzov reaction. Additionally, AMBPi 3c may not be stable enough and seems to lead to the formation of 5c, as previously described in the literature.[43]

Finally, the optimization of the cascade reaction showed the potent use of various commercially available and freshly prepared aliphatic and aromatic N,O-bis(trimethylsilyl)imidates 1a-c as silylating agent. In addition, the Lewis acid screening highlighted zinc iodide as the best compromise in terms of reactivity and reaction time.

2.2. NMR monitoring and purification details

As mentioned earlier, ³¹P and ³¹P {¹H} NMR experiments are routinely performed to follow the course of the reactions implying phosphorus derivatives. Figure 4 displays the optimized cascade reaction monitoring of the various phosphorus intermediate species.

The rapid silylation of H₃PO₂ was observed by the disappearance of its signal at 12.8 ppm for the benefit of a new signal at 141.6 ppm in the trivalent phosphorus region that confirmed the formation of BTSP as expected (Figure 4, spectra (b) versus (a)). After refluxing 1.5 hours, the NMR monitoring indicated the complete conversion of BTSP by its missing signal at 141.6 ppm and the appearance of several peaks in the P(III)/P(V) regions (142-147 ppm and 33-36 ppm) related to P(III)-TMS₃-3a and TMS₃-3a respectively (Figure 4, spectra (c) versus (b)). After methanolysis, a major signal remained at 22.0 ppm which matches the acidic form H₃-3a of AMBPi 3a (Figure 4, spectra (d) versus (c)). It was noted that small amounts of H₃PO₂ and H₃PO₃ were also generated. The pH adjustment at 10 conducted to AMBPi 3a as a disodium salt and concomitantly the partial precipitation of the zinc salt which was eliminated by centrifugation. Then, successive washes by ethyl acetate (with 0-10% ethanol) and methanol enabled to recover the excess of amide, and to discard both NaH₂PO₂ and Na₂HPO₃ respectively. The residual zinc salts were removed thanks to a cation-exchange resin.

The effectiveness of zinc elimination was verified by ICP-AES analysis. Finally, the disodium salt of AMBPi 3a was isolated after lyophilization as a pure form (Figure 4, spectra (e) versus (d)).

2.3. Viability considerations of the cascade reaction

The cascade reaction appears to be an interesting and straightforward method for accessing AMBPi scaffolds; however, 2 equivalents of silylimidates 1 are required for the silylation step, but only 0.5 equivalent of the silylamide generated in the first step participates in the sila-Arbuzov reaction. To overcome this significant drawback, we focused on retrieving the excess of amides initially used for the formation of the silylimidates (Scheme 2, (1)). Several attempts enabled us to finally recover up to 90 % of the resulting amides during the purification procedure which could be reused for the same reaction.

We also wondered if the reaction could proceed via a direct silylation of H₃PO₂ in the presence of Et₃N/TMSOTf followed by the addition of an amide in the presence of TMSOTf. Here, TMSOTf will both play the role of silylating agent and Lewis acid (Scheme 2, (b)). First, hypophosphorous acid, and 2 equivalents of triethylamine/TMSOTf were mixed at 0 °C. Unfortunately, the silylation was partial even after an extended period. To achieve completion, an additional equivalent of Et₃N/TMSOTf was added, leading to a 30-minute reaction time. However, this approach resulted in a significant amount of HP(O)(OTMS)₂ due to the oxidation of BTSP. In the second step, acetamide and TMSOTf were subsequently introduced at 0 °C.

Unfortunately, no AMBPi derivative was formed, and the reaction only resulted in the disproportionation of BTSP, giving hypophosphorous and phosphorous acids.

As a final attempt, we independently synthesized 2a and BTSP in the presence of triethylamine and TMSOTf, which were subsequently mixed together (Scheme 2, (c)). Alas, the reaction did not occur under these conditions.

These assays validated the viability of the cascade reaction we proposed. Moreover, the excess of amide can be successfully recovered, thus limiting its impact on the reaction implementation.

2.3. Scope of the cascade reaction

The scope of the cascade reaction was carried out in the presence of various prepared aliphatic and aromatic bis(trimethylsilyl)imidates 1a-l, hypophosphorous acid and zinc iodide as Lewis acid (Scheme 3). As a general trend, all imidates enabled to promote the silylation of H₃PO₂ into BTSP efficiently.

We were pleased to observe that the reaction was successful with aliphatic imidates bearing a longer chain 1b and 1d. In these cases, the corresponding AMBPi 3b and 3d were obtained in good conversions and isolated yields after purification. Although bis(trimethylsilyl)trifluoroacetimidate 1e can properly promote the silylation of H₃PO₂, the sila-Arbuzov reaction did not happen. It was only noted the oxidation of BTSP.

Concerning the use of aromatic bis(trimethylsilyl)imidate derivatives, the reactivity of para-substituted aromatic imidate derivatives was evaluated under the previous optimized conditions. The substitution at the para- position by a methoxy- group had little influence on the course of the reaction which conducted to AMBPi 3f in similar yield than 3c. When the reaction was performed with a para-substituted methyl moiety on silylamide 2i, the yield for the formation of 3i surprisingly decreased.

However, meta-substituted methyl aromatic amide 2j properly underwent the sila- Arbuzov in good conversion and isolated yield for 3j. The reaction was also carried out with electrowithdrawing para-fluoro and para-trifluoromethyl substituted groups on amides 2g and 2h. Although the conversion into 3g reached 60 %, the isolated yield dropped to 29 % due to its oxidation during the purification. Moreover, 3 h was not produced as only the disproportionation of BTSP took place.

The introduction of ortho- substituted methyl group and heteroaromatic moiety on aromatic imidates 2k and 2l was also considered. In these cases, only AMBPi 3l was generated in good conversion and isolated yield, probably due to steric hindrance of substrate 2k.

It was noted that α-aminophosphinates 5c,f,g,i,j were detected after methanolysis. According to NMR spectra, these compounds represented 10 to 15 % proportion (³¹P NMR) of crude products. This observation could justify the lower yields obtained for these AMBPi 3c,f,g,i,j.

Even if the results were moderate for some substituted aromatic AMBPi, this cascade reaction represents the first example of AMBPi which displays alkyl or aromatic groups at the methylene carbon on α-aminomethylenebisphosphinates.

3. Materials and Methods

3.1. General informations

Reagents were purchased from usual commercial suppliers (Sigma-Aldrich, Alfa Aesar, Acros Organics) and used as delivered. Triethylamine was distillated and stored over KOH under argon. All solvents were extra-dried grade prior used. N,O-bis(trimethylsilyl)acetamide (BSA) was purchased from Alfa Aesar (batch number: 10186753). Anhydrous H₃PO₂ was dehydrated from commercially available aqueous solution of H₃PO₂ (50% w/w) according to the procedure reported by Montchamp et al [53] Reactions requiring inert conditions were carried out in flame-dried glassware under an argon atmosphere. The solvents were degassed by argon bubbling for 30 minutes.

NMR spectra were recorded at 20 °C on a Bruker Avance-III-400 spectrometer (¹H: 400 MHz, ¹³C: 101 MHz, ³¹P: 162 MHz, ¹⁹F: 377 MHz). Chemical shifts (δ) were given in ppm, the number of protons (n) for a given resonance was indicated by n H and coupling constants J in Hz. ¹H NMR spectra were calibrated on non-deuterated solvent residual peak (H₂O: 4.79 ppm) while H₃PO₄ (85% in water) was used as an external standard for ³¹P NMR. The following abbreviations were used for ¹H, ¹³C, ³¹P and ¹⁹F NMR spectra to indicate the signal multiplicity: s (singlet), d (doublet), t (triplet), dd (doublet of doublets), dm (doublet of multiplet), m (multiplet), dq (doublet of quartets) and ddq (doublet of doublets of quartets). All ¹³C NMR spectra were measured with ¹H decoupling while ³¹P and ¹⁹F NMR spectra were measured with ¹H coupling and ¹H decoupling. ¹H experiments with water presaturation were performed with D₁ = 2 s and 128 scans. The reactions were followed by ³¹P and ³¹P{¹H} NMR experiments (the spectra were recorded without lock and shims). All NMR peak assignments were performed thanks to 2D NMR COSY, HMQC and HMBC experiments. High-resolution mass spectra (HRMS) were performed on a Bruker maXis mass spectrometer in negative (ESI-) mode (ESI) by the "Fédération de Recherche" ICOA/CBM (FR2708) platform. MS analyses were performed using a QTOF Impact HD mass spectrometer equipped with the electrospray (ESI) ion source (Bruker Daltonics). The instrument was operated in the negative mode with an ESI source on a Q-TOF mass spectrometer with an accuracy tolerance of 2 ppm. Samples were diluted with acetonitrile and water (15:85) and were analyzed by mass spectrometry in continuous infusion using a syringe pump at 200 µL/min. The mass profiles obtained by ESI-MS were analyzed using Data Analysis software (Bruker Daltonics). ICP-AES analyses were performed by “plateforme Analytiques des Inorganiques” IPHC UMR7178 on Varian 720ES.

3.2. General procedure for the cascade synthesis of Aminomethylenebisphosphinates 3a-l

To a dry and argon flushed 100 mL three-necked flask, equipped with a thermometer, an argon inlet and a septum, were successively introduced the corresponding amide 1a-l (15.00 mmol, 1.00 equiv.), anhydrous pentane (34.00 mL), anhydrous dichloromethane (1.50 mL) and triethylamine (33.00 mmol, 5.58 mL, 2.20 equiv.). Trimethylsilyltrifloromethanesulfonate (33.00 mmol, 5.73 mL, 2.20 equiv.) was added dropwise at 0 °C and the mixture was stirred for 30 minutes at room temperature. The lower phase obtained during the process was eliminated. Then, the solvent was evaporated under reduced pressure. The imidates 2a-l were used in the next step without further purification.

To another dry and argon flushed 25 mL three necked flask equipped with a thermometer, a reflux condenser with an argon inlet and a septum was added anhydrous hypophosphorous acid (5.00 mmol, 0.330 g, 0.50 equiv.) and anhydrous tetrahydrofuran (1.00 mL) under argon atmosphere. The synthesized imidates 2a-l (10.00 mmol, 2.00 equiv.) were added dropwise at 0 °C and the mixture was stirred for 40 minutes. The reaction conversion was monitored by ³¹P NMR. A solution of zinc iodide (2.50 mmol, 0.750 g, 0.50 equiv.) in anhydrous tetrahydrofuran (4.00 mL) was added dropwise at 0°C and the mixture was stirred under reflux condition. The reaction conversion was also monitored by ³¹P NMR upon completion. Then, anhydrous methanol (3.00 mL) was added dropwise at 0°C. The solvent was evaporated, and the crude compound was dissolved in minimum of water (2.00 mL) and an aqueous solution of sodium hydroxide (0.50 M, 8.00 mL) was added carefully to adjust pH to 10.00. The mixture was centrifugated to partially discard precipitated zinc salts. The filtrate was then washed with ethyl acetate (5 x 5.00 mL) (with 0-10 % ethanol) and methanol (10 x 2.00 mL) to eliminate the excess of amides 1a-l and phosphorous acid respectively. In addition, a cation-exchange resin was used to eliminate the residual zinc salts. Finally, the solution was lyophilized to afford the pure AMBPi 3 as a disodium salt.

3.2. Spectral data of aminomethylenebisphosphinates 3a-l

1-aminoethane-1,1-bis(H-phosphinate) disodium salts 3a. White powder. 425 mg, 79 % yield. ³¹P {¹H} NMR (162 MHz, D₂O) δ 28.5 (s). ³¹P NMR (162 MHz, D₂O) δ 28.5 (dm, ¹J_P-H= 524.9 Hz). ¹H NMR (400 MHz, D₂O) δ 6.80 (dt, ¹J_P-H= 525.3 Hz, ²J= 11.7 Hz, 2H), 1.19 (t, ²J_P-H = 15.8 Hz, 3H). ¹³C NMR (101 MHz, D₂O) δ 52.2 (t, ¹J_P-C= 89.4 Hz), 15.0. MS (ESI-) m/z 171.99 [M-H]^-, 193.97 [M-2H+Na]^-, 153.98 [M-H-H₂O]^-. HRMS (ESI-) m/z: [M-H]^- Calcd. for [C₂H₈NO₄P₂]: 171.9934, found: 171.9934.

1-amino-1-propylmethane-1,1-bis(H-phosphinate) disodium salts 3b. White powder. 359 mg, 60 % yield. ³¹P {¹H} NMR (162 MHz, D₂O) δ 28.1 (s). ³¹P NMR (162 MHz, D₂O) δ 28.1 (dp, ¹J_P-H= 523.6 Hz, ²J= 13.4 Hz). ¹H NMR (400 MHz, D₂O) δ 6.84 (dt, ¹J_P-H= 523.5 Hz, ²J= 12.1 Hz, 2H), 1.72-1.55 (m, 2H), 1.54-1.39 (m, 2H), 0.88 (t, J= 7.2 Hz, 3H). ¹³C NMR (101 MHz, D₂O) δ 55.3 (t, ¹J_P-C= 89.0 Hz, 33.1, 16,6 (t, ²J_P-C = 6.8 Hz), 14.3. MS (ESI-) m/z 200.02 [M-H]^-, 222.00 [M-2H+Na]^-, 182.01 [M-H-H₂O]^-, 134.04 [M-H-H₃PO₂]^-. HRMS (ESI-) m/z: [M-H]^- Calcd. for [C₄H₁₂NO₄P₂]: 200.0247, found: 200.0247.

1-amino-1-phenylmethane-1,1-bis(H-phosphinate) disodium salts 3c. White powder. 500 mg, 72 % yield. ³¹P {¹H} NMR (162 MHz, D₂O) δ 26.8 (s). ³¹P NMR (162 MHz, D₂O) δ 26.8 (dt, ¹J_P-H= 538.6 Hz, J= 12.1 Hz). ¹H NMR (400 MHz, D₂O) δ 7.43 (d, ³J_P-H = 8.1 Hz, 2H), 7.32 (t, ⁴J_P-H= 7.6 Hz, 2H), 7.23 (t, ¹J_P-H= 7.6 Hz, 1H), 6.87 (dt, ¹J_P-H= 539.0 Hz,²J = 10.3 Hz, 2H). ¹³C NMR (101 MHz, D₂O) δ 164.0, 128.5, 127.0, 126.0, 60.5 (t, ¹J_P-C= 86.0 Hz. MS (ESI-) m/z 234.00 [M-H]^-, 215.99 [M-H-H₂O]^-, 170.04 [M-H-HPO₂]^-. HRMS (ESI-) m/z: [M-H]^- Calcd. for [C₇H₁₀NO₄P₂]: 234.0090, found: 234.0090.

1-amino-1-butylmethane-1,1-bis(H-phosphinate) disodium salts 3d White powder, 417 mg, 65 % yield. ³¹P {¹H} NMR (162 MHz, D₂O) δ 27.9 (s). ³¹P NMR (162 MHz, D₂O) δ 27.9 (dp, ¹J_P-H= 523.8 Hz, ²J= 13.2 Hz). ¹H NMR (400 MHz, D₂O) δ 6.85 (dt, ¹J_P-H= 524.3 Hz, ²J= 11.9 Hz, 2H), 1.73-1.62 (m, 2H), 1.48-1.40 (m, 2H), 1.28 (hex., J= 7.3 Hz, 2H), 0.86 (t, J= 7.3 Hz, 3H). ¹³C NMR (101 MHz, D₂O) δ 55.2 (t, ¹J_P-C= 88.6 Hz), 30.45 (C₂), 25.1 (t, ³J_P-C = 6.7 Hz), 23.0, 13.1. MS (ESI-) m/z 214.04 [M-H]^-, 236.02 [M-2H+Na]^-, 196.03 [M-H-H₂O]^-.HRMS (ESI-) m/z: [M-H]^- Calcd. for [C₅H₁₄NO₄P₂]: 214.0403, found: 214.0403.

1-amino-1-(4-methoxyphenyl)methane-1,1-bis(H-phosphinate) disodium salts 3f. White powder. 0.483 mg, 65 % yield. ³¹P {¹H} NMR (162 MHz, D₂O) δ 26.8 (s). ³¹P NMR (162 MHz, D₂O) δ 26.8 (dt, ¹J_P-H= 538.7 Hz, ²J= 12.4 Hz). ¹H NMR (400 MHz, D₂O) 7.47 (d, ³J= 8.9 Hz, 2H), 7.02 (d, ³J_P-H= 8.5 Hz, 2H), δ 6.95 (dt, ¹J_P-H= 537.9 Hz, ²J= 11.1 Hz, 2H), 3.81 (s, 3H). ¹³C NMR (101 MHz, D₂O) δ 157.8 (t, J = 2.4 Hz), 128.2, 127.4 (t, ³J_P-C= 4.9 Hz), 114.0, 59.8 (t, ¹J_P-C= 86.8 Hz), 55.3. MS (ESI-) m/z 264.02 [M-H]^-, 286.00 [M-2H+Na]^-, 246.01 [M-H-H₂O]^-, 200.05 [M-H-HPO₂]^-. HRMS (ESI-) m/z: [M-H]^- Calcd. for [C₈H₁₂NO₅P₂]: 264.0196, found: 200.0204.

1-amino-1-(4-fluorophenyl)methane-1,1-bis(H-phosphinate) disodium salts 3g. White powder. 220 mg, 29 % yield. ³¹P {¹H} NMR (162 MHz, D₂O) δ 26.4 (s). ³¹P NMR (162 MHz, D₂O) δ 26.4 (dt, ¹J_P-H= 538.6 Hz, J= 11.1 Hz). ¹⁹P NMR (377 MHz, D₂O) δ 116.8 (m). ¹H NMR (400 MHz, D₂O) δ 7.57-7.46 (m, 2H), 7.14 (t, ⁴J_P-H= 8.8 Hz, 2H), 6.96 (dt, ¹J_P-H= 537.4 Hz, ¹J_P-H = 10.8 Hz, 2H). ¹³C NMR (101 MHz, D₂O) δ 160.7 (dt, ¹J_C-F= 243.2 Hz, ⁴J_P-C= 2.8 Hz), 131.6-131.5 (m), 127.8-127.7 (m), 115.2, 115.0, 60.0 (t, ¹J_P-C= 86.0 Hz). MS (ESI-) m/z 252.00 [M-H]^-, 273.98 [M-2H+Na]^-, 233.99 [M-H-H₂O]^-, 188.03 [M-H-HPO₂]^-. HRMS (ESI-) m/z: [M-H]^- Calcd. for [C₇H₉FNO₄P₂]: 251.9996, found: 251.9996.

1-amino-1-(4-tolyl)methane-1,1-bis(H-phosphinate) disodium salts 3i. White powder. 250 mg, 35 % yield. ³¹P {¹H} NMR (162 MHz, D₂O) δ 26.8 (s). ³¹P NMR (162 MHz, D₂O) δ 26.8 (dt, ¹J_P-H= 539.8 Hz, J= 12.0 Hz). ¹H NMR (400 MHz, D₂O) δ 7.46-7.40 (m, 2H), 7.26-7.22 (m, 2H), 6.96 (dt, ¹J_P-H= 538.2 Hz, ²J= 10.8 Hz, 2H), 2.30 (s, 3H). ¹³C NMR (101 MHz, D₂O) δ 137.0, 132.6, 129.1, 126.05 (t, ³J_P-C= 4.8 Hz), 60.2 (t, ¹J_P-C= 86.5 Hz), 20.1. MS (ESI-) m/z 248.02 [M-H]^-, 270.00 [M-H-H₂O]^-, 184.05 [M-H-HPO₂]^-. HRMS (ESI-) m/z: [M-H]^- Calcd. for [C₈H₁₂FNO₄P₂]: 248.0247, found: 248.0247.

1-amino-1-(3-tolyl)phenyl)methane-1,1-bis(H-phosphinate) disodium salts 3j. White powder. 400 mg, 57 % yield. ³¹P {¹H} NMR (162 MHz, D₂O) δ 26.8 (s). ³¹P NMR (162 MHz, D₂O) δ 26.8 (dt, ¹J_P-H= 542.3 Hz, J= 11.5 Hz). ¹H NMR (400 MHz, D₂O) δ 7.36 (s, 1H), 7.30-7.29 (m, 2H), 7.15 (s, 1H), 6.96 (dt, ¹J_P-H= 538.9 Hz, ²J = 11.8 Hz, 2H), 2.32 (s, 3H). ¹³C NMR (101 MHz, D₂O) δ 138.4, 135.8 (t, ⁴J_P-C = 2.3 Hz), 128.4, 127.6, 126.7 (t, ³J_P-C = 4.9 Hz), 123.0 (t, ³J_P-C = 5.0 Hz), 60.5 (t, ¹J_P-C = 86.1 Hz), 20.7. MS (ESI-) m/z 248.02 [M-H]^-, 270.01 [M-2H+Na]^-, 230.01 [M-H-H₂O]^-, 184.05 [M-H-HPO₂]^-. HRMS (ESI-) m/z: [M-H]^- Calcd. for [C₈H₁₂NO₄P₂]: 248.0247, found: 248.0247.

1-amino-1-(2-thienyl)ethane-1,1-bis(H-phosphinate) disodium salts 3l. White powder. 437 mg, 59 % yield. ³¹P {¹H} NMR (162 MHz, D₂O) δ 26.5 (s). ³¹P NMR (162 MHz, D₂O) δ 26.5 (dm, ¹J_P-H= 531.6 Hz). ¹H NMR (400 MHz, D₂O) δ 7.28-7.27 (m, 1H, H₆), 6.99-6.97 (m, 2H), 6.83 (dt, ¹J_P-H = 530.6 Hz, ²J= 11.8 Hz, 2H), 3.26 (t, J= 12.7 Hz, 2H). ¹³C NMR (101MHz, D₂O) δ 136.9 (t, ³J_P-C = 9.1 Hz), 128.5, 126.9, 125.1, 55.1 (t, ¹J_P-C = 89.3 Hz), 29.8. MS (ESI-) m/z 253.98 [M-H]^-, 275.96 [M-2H+Na]^-, 253.97 [M-H-H₂O]^-. HRMS (ESI-) m/z: [M-H]^- Calcd. for [C₆H₁₀NO₄P₂S]: 253.9811, found: 253.9811.

4. Conclusions

In this study, we have established a cascade reaction involving the silylation of hypophosphorous acid by a N,O-bis(trimethylsilyl)imidate, leading to the formation of bis(trimethylsilyl)phosphonite (BTSP) and a N-silylamide. The latter can subsequently undergo nucleophilic attack of BTSP through a sila-Arbuzov reaction which is mediated by zinc iodide as Lewis acid. This approach relies on an unexpected result as our initial attempt was to investigate the reactivity of nitriles in the presence of BTSP and a Lewis acid. We present a detailed methodology to propose a novel access to AMBPi scaffolds which have been understudied in the literature. The screening of Lewis acid has highlighted zinc iodide as the best promoter for the sila-Arbuzov reaction. Consequently, we successfully synthesized various AMBPi 3a-l in moderate to good yields. Better results were obtained in aliphatic series and will enable us to extend this method to more functionalized AMBPi, analogous to aminomethylenebisphosphonates which have demonstrated relevant biological activities.