Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Phytochemical Screening and Evaluation of Contractile Effects of the Aqueous and Hydroethanolic Extracts of Anastatica hierochuntica L. (Brassicaceae) on Pregnant Mice Isolated Myometrium

Wendkouni Leila Marie Esther Belem-Kabré
* ,
Mathieu Nitièma
,
Blondine Saïdath Odjo
,
Oumarou Maman Noura
,
Boubacar Yaro
,
Boukaré Kaboré
,
Gaétan Somda
,
Kadiatou Tata Traoré
,
Moumouni Koala
,
Sylvain Ilboudo
,
Moussa Ouédraogo
,
Elie Kabré
,
Noufou Ouédraogo
Version 1 : Received: 14 July 2023 / Approved: 17 July 2023 / Online: 17 July 2023 (10:41:17 CEST)

How to cite: Belem-Kabré, W.L.M.E.; Nitièma, M.; Odjo, B.S.; Maman Noura, O.; Yaro, B.; Kaboré, B.; Somda, G.; Traoré, K.T.; Koala, M.; Ilboudo, S.; Ouédraogo, M.; Kabré, E.; Ouédraogo, N. Phytochemical Screening and Evaluation of Contractile Effects of the Aqueous and Hydroethanolic Extracts of Anastatica hierochuntica L. (Brassicaceae) on Pregnant Mice Isolated Myometrium. Preprints 2023, 2023071102. https://doi.org/10.20944/preprints202307.1102.v1 Belem-Kabré, W.L.M.E.; Nitièma, M.; Odjo, B.S.; Maman Noura, O.; Yaro, B.; Kaboré, B.; Somda, G.; Traoré, K.T.; Koala, M.; Ilboudo, S.; Ouédraogo, M.; Kabré, E.; Ouédraogo, N. Phytochemical Screening and Evaluation of Contractile Effects of the Aqueous and Hydroethanolic Extracts of Anastatica hierochuntica L. (Brassicaceae) on Pregnant Mice Isolated Myometrium. Preprints 2023, 2023071102. https://doi.org/10.20944/preprints202307.1102.v1

Abstract

Anastatica hierochuntica L. (Brassicaceae) has been claimed to treat various conditions including complicated childbirth. The principal objective of this research was to provide scientific evidence on the contractile effect of the aqueous and hydroethanolic extracts of the whole plant from A. hierochuntica during labor. Qualitative and quantitative methods helped identify and quantify the phytoconstituents in both extracts. The antioxidant activity was studied using four standard methods. The inhibitory power of the extracts on pro-inflammatory enzymes such as 15-lipoxygenase and phospholipase A2 was determined. The contractile effects of the extracts were evaluated using isolated pregnant mice uteri. Steroids, triterpenoids, flavonoids, tannins, coumarins, and reduced compounds were identified in both extracts. The extracts demonstrated the ability to reduce oxidative stress. The hydroethanolic extract displayed the most significant anti-lipoxygenase activity with an IC50 value of 55.82 ± 1.15 µg/mL. The lethal dose (LD50) of extracts was estimated to be superior to 2000 mg/kg bw. The ability of the samples to produce one cm amplitude of contraction at 50% effective concentrations (EC50) was 1.51 ± 0.08 mg/mL for aqueous extract and 3.57 ± 0.61 mg/mL for hydroethanolic extract. These findings could provide evidence for using Anastatica hierochuntica to augment labor.

Keywords

Anastatica hierochuntica L; Phytoconstituents; Antioxidant; Anti-inflammatory; Acute toxicity; Uterine contractility; labor

Subject

Medicine and Pharmacology, Pharmacology and Toxicology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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