Chiticaru, E.A.; Damian, C.M.; Pilan, L.; Ioniță, M. Label-Free DNA Biosensor Based on Reduced Graphene Oxide and Gold Nanoparticles. Biosensors2023, 13, 797.
Chiticaru, E.A.; Damian, C.M.; Pilan, L.; Ioniță, M. Label-Free DNA Biosensor Based on Reduced Graphene Oxide and Gold Nanoparticles. Biosensors 2023, 13, 797.
Chiticaru, E.A.; Damian, C.M.; Pilan, L.; Ioniță, M. Label-Free DNA Biosensor Based on Reduced Graphene Oxide and Gold Nanoparticles. Biosensors2023, 13, 797.
Chiticaru, E.A.; Damian, C.M.; Pilan, L.; Ioniță, M. Label-Free DNA Biosensor Based on Reduced Graphene Oxide and Gold Nanoparticles. Biosensors 2023, 13, 797.
Abstract
Currently available DNA detection techniques frequently require compromises between simplicity, speed, accuracy, and cost. Here, we propose a simple, label-free and cost-effective DNA detection platform developed at screen printed carbon electrodes (SPCEs) modified with reduced graphene oxide (RGO) and gold nanoparticles (AuNPs). The preparation of the detection platform involved a two-step electrochemical procedure based on GO reduction onto SPCEs followed by the electrochemical reduction of HAuCl4 to facilitate the post-grafting reaction with AuNPs. The final sensor was fabricated by the simple physical adsorption of a single-stranded DNA (ssDNA) probe onto AuNPs-RGO/SPCE electrode. Each preparation step was confirmed by morphological and structural characterization using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy, respectively. Furthermore, the electrochemical properties of the modified electrodes have been investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results demonstrated that the introduction of AuNPs onto RGO/SPCEs led to an enhancement in surface conductivity, characteristic that favored an increased sensitivity in detection. The detection process relied on the change in the electrochemical signal induced by the binding of target DNA to the bioreceptor, and was monitored particularly by the change in the charge transfer resistance of a [Fe(CN)6]4−/3− redox couple added in the test solution.
Keywords
biosensor; electrochemistry; graphene; gold nanoparticles; DNA detection
Subject
Chemistry and Materials Science, Electrochemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
