PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Rapid-Test Kit Creation of Cardiac Troponin I, A Reliable Biosensor for Naked-Eye Detection Method in Daily Usage and Pharmacokinetic Studies for myocardial Infarction, Systemic Amyloidosis, and Cardiovascular Disease
Version 1
: Received: 14 July 2023 / Approved: 14 July 2023 / Online: 14 July 2023 (15:25:47 CEST)
How to cite:
Hsieh, Y.; Lin, K. Rapid-Test Kit Creation of Cardiac Troponin I, A Reliable Biosensor for Naked-Eye Detection Method in Daily Usage and Pharmacokinetic Studies for myocardial Infarction, Systemic Amyloidosis, and Cardiovascular Disease. Preprints2023, 2023071025. https://doi.org/10.20944/preprints202307.1025.v1
Hsieh, Y.; Lin, K. Rapid-Test Kit Creation of Cardiac Troponin I, A Reliable Biosensor for Naked-Eye Detection Method in Daily Usage and Pharmacokinetic Studies for myocardial Infarction, Systemic Amyloidosis, and Cardiovascular Disease. Preprints 2023, 2023071025. https://doi.org/10.20944/preprints202307.1025.v1
Hsieh, Y.; Lin, K. Rapid-Test Kit Creation of Cardiac Troponin I, A Reliable Biosensor for Naked-Eye Detection Method in Daily Usage and Pharmacokinetic Studies for myocardial Infarction, Systemic Amyloidosis, and Cardiovascular Disease. Preprints2023, 2023071025. https://doi.org/10.20944/preprints202307.1025.v1
APA Style
Hsieh, Y., & Lin, K. (2023). Rapid-Test Kit Creation of Cardiac Troponin I, A Reliable Biosensor for Naked-Eye Detection Method in Daily Usage and Pharmacokinetic Studies for myocardial Infarction, Systemic Amyloidosis, and Cardiovascular Disease. Preprints. https://doi.org/10.20944/preprints202307.1025.v1
Chicago/Turabian Style
Hsieh, Y. and Kuan-Jiuh Lin. 2023 "Rapid-Test Kit Creation of Cardiac Troponin I, A Reliable Biosensor for Naked-Eye Detection Method in Daily Usage and Pharmacokinetic Studies for myocardial Infarction, Systemic Amyloidosis, and Cardiovascular Disease" Preprints. https://doi.org/10.20944/preprints202307.1025.v1
Abstract
Myocardial infarction (MI) is a serious cardiovascular disease that can lead to death. Cardiac troponin I (cTnI) is a biomarker of MI in the circulation system. For detection cTnI protein, many method suffer the same problem, time-consuming. Main drawbacks are time consuming, have tedious operation, require an expensive reader for translating signals, and require non-accessible reagents. Our goal is to create a kit for cTnI protein testing, that the result can easily be read by color difference. The main breakthrough is, the resolution between different concentration can be told by eyes. The result will be future used to create a commercial, potable, convenient, daily-used rapid-test kit. In this study, a cTnI biosensor is proposed that can be read by the naked eye, performs diagnosis based on the pattern color, does not require a reader machine, is easy to operate, and is portable. Our work proposed a device that shortens the diagnosis time, has a quantitative analysis range of 0.32 –200 ng/mL in the human serum matrix, has a 0.32 ng/mL limit of detection (LOD), and shows many advantages comparing to traditional cTnI ELISA plate.
Chemistry and Materials Science, Applied Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
