Version 1
: Received: 10 July 2023 / Approved: 11 July 2023 / Online: 12 July 2023 (09:18:55 CEST)
How to cite:
Ng, W. Detection of Two Phylogenetic Clusters in Helicobacter pylori 23S rRNA Phylogeny Suggests Emergence of Two Distinct Phylotype of the Species. Preprints2023, 2023070751. https://doi.org/10.20944/preprints202307.0751.v1
Ng, W. Detection of Two Phylogenetic Clusters in Helicobacter pylori 23S rRNA Phylogeny Suggests Emergence of Two Distinct Phylotype of the Species. Preprints 2023, 2023070751. https://doi.org/10.20944/preprints202307.0751.v1
Ng, W. Detection of Two Phylogenetic Clusters in Helicobacter pylori 23S rRNA Phylogeny Suggests Emergence of Two Distinct Phylotype of the Species. Preprints2023, 2023070751. https://doi.org/10.20944/preprints202307.0751.v1
APA Style
Ng, W. (2023). Detection of Two Phylogenetic Clusters in Helicobacter pylori 23S rRNA Phylogeny Suggests Emergence of Two Distinct Phylotype of the Species. Preprints. https://doi.org/10.20944/preprints202307.0751.v1
Chicago/Turabian Style
Ng, W. 2023 "Detection of Two Phylogenetic Clusters in Helicobacter pylori 23S rRNA Phylogeny Suggests Emergence of Two Distinct Phylotype of the Species" Preprints. https://doi.org/10.20944/preprints202307.0751.v1
Abstract
Phylogenetic tree analysis is commonly used to understand the evolutionary distance between different species or strains of the same species. This work originally aimed to gain an understanding and visual depiction of the evolutionary distance in 23S rRNA gene of different Helicobacter pylori strains. Using an in-house MATLAB multiple sequence alignment and phylogenetic tree analysis software, the phylogenetic tree constructed for 23S rRNA gene reveals two major lineages with further subdivisions in each cluster. This suggests two major evolutionary forces driving the evolution of the 23S rRNA gene. In comparison, there is relatively short evolutionary distance in the 16S rRNA gene phylogenetic tree of H. pylori, suggesting lack of major evolutionary forces driving mutational changes of the gene. Overall, the data suggests that the large ribosome subunit which houses the 23S rRNA of H. pylori is undergoing significant conformational changes that may be a response to the frequent use of various antibiotics that target the ribosome of H. pylori.
Keywords
Helicobacter pylori; large ribosome subunit; small ribosome subunit; 16S rRNA gene; 23S rRNA gene
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
