Flores-Rábago, K.M.; Rivera-Mendoza, D.; Vilchis-Nestor, A.R.; Juarez-Moreno, K.; Castro-Longoria, E. Antibacterial Activity of Biosynthesized Copper Oxide Nanoparticles (CuONPs) Using Ganoderma sessile. Antibiotics2023, 12, 1251.
Flores-Rábago, K.M.; Rivera-Mendoza, D.; Vilchis-Nestor, A.R.; Juarez-Moreno, K.; Castro-Longoria, E. Antibacterial Activity of Biosynthesized Copper Oxide Nanoparticles (CuONPs) Using Ganoderma sessile. Antibiotics 2023, 12, 1251.
Flores-Rábago, K.M.; Rivera-Mendoza, D.; Vilchis-Nestor, A.R.; Juarez-Moreno, K.; Castro-Longoria, E. Antibacterial Activity of Biosynthesized Copper Oxide Nanoparticles (CuONPs) Using Ganoderma sessile. Antibiotics2023, 12, 1251.
Flores-Rábago, K.M.; Rivera-Mendoza, D.; Vilchis-Nestor, A.R.; Juarez-Moreno, K.; Castro-Longoria, E. Antibacterial Activity of Biosynthesized Copper Oxide Nanoparticles (CuONPs) Using Ganoderma sessile. Antibiotics 2023, 12, 1251.
Abstract
Copper oxide nanoparticles (CuONPs) were synthesized using an eco-friendly method and their antimicrobial and biocompatibility properties were determined. The supernatant and extract of the fungus Ganoderma sessile yielded small, quasi-spherical NPs with an average size of 4.5 ± 1.9 nm and 5.2 ± 2.1 nm, respectively. CuONPs showed antimicrobial activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Minimum inhibitory concentration (MIC) for E. coli and S. aureus was 15.9 µg/mL for nanoparticles (NPs) using the supernatant (CuONPs-S) and 16.5 µg/ml for NPs using the extract (CuONPs-E). Lower concentrations were required for P. aeruginosa inhibition. Ultrastructural analysis revealed the presence of the small CuONPs all through the bacterial cells. Finally, the toxicity of CuONPs was analyzed in three mammalian cell lines: hepatocytes (AML-12), macrophages (RAW 264.7) and kidney (MDCK). Low concentrations (<15 µg/ml) of CuONPs-E were non-toxic to kidney cells and macrophages, and the hepatocytes were the most susceptible to CuONPs-S. The results obtained suggest that the CuONPs synthesized using the extract of the fungus G. sessile, could be further evaluated for the treatment of superficial infectious diseases.
Keywords
green method; copper oxide nanoparticles; antibacterial; ultrastructure
Subject
Chemistry and Materials Science, Nanotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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