PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Investigation of Wound Healing and Anticancer Properties of in vitro Grown Sesame Plant Extract in L929, A549, and MCF-7 Cell Lines and Transcriptomic Analyses of Cas3, Cas9, and BCL-XL Genes in the Sesame Extract Treated Cells
Version 1
: Received: 26 June 2023 / Approved: 27 June 2023 / Online: 27 June 2023 (09:08:02 CEST)
How to cite:
Yeniocak, S.; Galatalı, S.; Demir, İ.; Uğur, A.; Saraç, N.; Kaya, E. Investigation of Wound Healing and Anticancer Properties of in vitro Grown Sesame Plant Extract in L929, A549, and MCF-7 Cell Lines and Transcriptomic Analyses of Cas3, Cas9, and BCL-XL Genes in the Sesame Extract Treated Cells. Preprints2023, 2023061866. https://doi.org/10.20944/preprints202306.1866.v1
Yeniocak, S.; Galatalı, S.; Demir, İ.; Uğur, A.; Saraç, N.; Kaya, E. Investigation of Wound Healing and Anticancer Properties of in vitro Grown Sesame Plant Extract in L929, A549, and MCF-7 Cell Lines and Transcriptomic Analyses of Cas3, Cas9, and BCL-XL Genes in the Sesame Extract Treated Cells. Preprints 2023, 2023061866. https://doi.org/10.20944/preprints202306.1866.v1
Yeniocak, S.; Galatalı, S.; Demir, İ.; Uğur, A.; Saraç, N.; Kaya, E. Investigation of Wound Healing and Anticancer Properties of in vitro Grown Sesame Plant Extract in L929, A549, and MCF-7 Cell Lines and Transcriptomic Analyses of Cas3, Cas9, and BCL-XL Genes in the Sesame Extract Treated Cells. Preprints2023, 2023061866. https://doi.org/10.20944/preprints202306.1866.v1
APA Style
Yeniocak, S., Galatalı, S., Demir, İ., Uğur, A., Saraç, N., & Kaya, E. (2023). Investigation of Wound Healing and Anticancer Properties of <em>in vitro</em> Grown Sesame Plant Extract in L929, A549, and MCF-7 Cell Lines and Transcriptomic Analyses of <em>Cas3, Cas9</em>, and BCL-XL Genes in the Sesame Extract Treated Cells. Preprints. https://doi.org/10.20944/preprints202306.1866.v1
Chicago/Turabian Style
Yeniocak, S., Nurdan Saraç and Ergun Kaya. 2023 "Investigation of Wound Healing and Anticancer Properties of <em>in vitro</em> Grown Sesame Plant Extract in L929, A549, and MCF-7 Cell Lines and Transcriptomic Analyses of <em>Cas3, Cas9</em>, and BCL-XL Genes in the Sesame Extract Treated Cells" Preprints. https://doi.org/10.20944/preprints202306.1866.v1
Abstract
In addition to its use as a food additive, sesame is also very popular in conventional drugs because of its antifungal, anticancer, analgesic, antioxidant, and antiproliferative, vitamin B and E supplier, serum cholesterol and blood pressure-lowering, wound healing potential. In this study, the cytotoxicity, wound healing, and anticancer (antiproliferation) properties of the ex-tract obtained from in vitro cultures of Sesamum orientale L. cv. "Gökova" were investigated using in L929 fibroblast, MCF-7 breast, and A549 lung epithelial cell lines. In our study, the cisplatin was also used as a control group to compare the anticancer efficacy of our plant extract. The IC50 values obtained from cell treatments were 922.73 µg.ml-1 (plant extract) and 33.09 µg.ml-1 (cispla-tin) for A549 µg.ml-1, 1837.07 µg.ml-1 (plant extract) and 19.27 µg.ml-1 (cisplatin) for MCF-7, and 154.70 µg.ml-1 (plant extract) for L929, respectively. The subcytotoxic doses of the treated plant extract provided the healing of artificially created wounds on L929 fibroblast cell cultures within 48 hours. For the evaluation of the anticancer activity, it was also determined that transcriptomic analyzes of BCL-XL gene, which is negatively correlated with apoptotic pathway, and Cas3 and Cas9 genes, which are positively correlated with apoptotic pathway, showed a statistically sig-nificant increase in A549 and MCF-7 cell lines treated with plant extract or cisplatin. In the light of the results obtained from the present study, it was seen that sesame plant extract may have wound healing potential at decreasing doses and anticancer activity potential at increasing dos-es. The present study can be a useful resource for the development of a drug with wound healing and/or antiproliferative potential, with applications to be made in different cell lines in the future.
Keywords
Sesamum orientale L. cv. “Gökova”; L929 fibroblast cells; A549 lung epithelial cells; MCF-7 breast epithelial cells; Cisplatin; BCL-XL gene; Cas3 and Cas9 genes
Subject
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
