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This version is not peer-reviewed
Treatment | Abbreviation | Number of fish Challenged | Number of Total Dead Fish at 15 dpc | RPS (%) 15 dpc |
---|---|---|---|---|
Water based (W) | ||||
Control (Cell culture supernatant, L15) | W-C | 31 | 5 | |
Heat-killed vaccine | W-HKV | 27 | 2 | 54.6 |
Formalin-killed vaccine | W-FKV | 31 | 2 | 59.7 |
Oil-based (O) (MontanideTM ISA 763A VG) | ||||
Control (L15 + Oil adjuvant) | O-C | 32 | 8 | |
Heat-killed vaccine | O-HKV | 28 | 5 | 32.0 |
Formalin-killed vaccine | O-FKV | 24 | 9 | 0 |
Gene | Oligo Sequences | Annealing Temperature (oC) | Product Size (bp) | Reference |
---|---|---|---|---|
EF-1α | F-5’-CTACAGCCAGGCTCGTTTCG-3’ R-5’-CTTGTCACTGGTCTCCAGCA-3’ |
56 | 139 | [30] |
IgM | F-5’-GGATGACGAGGAAGCAGACT-3’ R-5’-CATCATCCCTTTGCCACTGG-3’ |
53 | 122 | [30] |
IgT | F-5’-TGACCAGAAATGGCGAAGTCTG-3’ R-5’-GTTATAGTCACATTCTTTAGAATTACC-3’ |
53 | 163 | [30] |
IgD | F-5′- AACACCACCCTGTCCCTGAAT-3′ R-5’-GGGTGAAAACCACATTCCAAC-3’ |
61 | 127 | [31] |
Time Points | Treatments | Head Kidney | Spleen | ||||
---|---|---|---|---|---|---|---|
IgM | IgT | IgD | IgM | IgT | IgD | ||
D7 | O-C | 1.07 ± 0.46 | 1.02 ± 0.20 | 1.07 ± 0.44 | 1.51 ± 1.60 | 1.20 ± 0.75 | 1.05 ± 0.38 |
O-HKV | 1.75 ± 0.78 | 3.14 ± 0.73↑ | 2.93 ± 1.24↑ | 1.14 ± 0.45 | 0.90 ± 0.49 | 1.00 ± 0.78 | |
O-FKV | 1.53 ± 0.47 | 1.61 ± 0.99 | 1.89 ± 0.11↑ | 1.51 ± 0.94 | 0.93 ± 0.75 | 2.04 ± 1.34 | |
W-C | 1.55 ± 1.77 | 1.48 ± 1.61 | 1.07 ± 0.44 | 1.72 ± 1.97 | 1.79 ± 2.09 | 1.24 ± 0.86 | |
W-HKV | 0.96 ± 0.74 | 0.55 ± 0.32 | 1.19 ± 0.86 | 1.04 ±1.02 | 1.09 ± 0.96 | 1.23 ± 0.86 | |
W-FKV | 6.00 ± 2.81↑ | 1.43 ± 0.85 | 3.80 ± 1.81↑ | 0.31 ± 0.12 | 0.38 ± 0.22 | 0.42 ± 0.16 | |
D14 | O-C | 1.58 ± 1.21 | 1.09 ± 0.48 | 1.01 ± 0.17 | 1.19 ± 0.70 | 1.67 ± 1.72 | 1.20 ± 0.89 |
O-HKV | 4.94 ± 4.11 | 0.77 ± 0.45 | 1.46 ± 0.92 | 1.09 ± 0.80 | 1.00 ± 0.67 | 1.22 ± 1.04 | |
O-FKV | 1.84 ± 2.25 | 0.37 ± 0.36 | 1.08 ±1.14 | 1.81 ± 0.87 | 1.11 ± 0.32 | 2.71 ± 1.71 | |
W-C | 1.44 ± 1.05 | 0.96 ± 0.80 | 1.53 ± 1.70 | 1.43 ± 1.19 | 2.31 ± 2.80 | 1.66 ± 1.88 | |
W-HKV | 1.81 ± 0.64 | 1.09 ± 0.53 | 1.35 ± 0.45 | 6.91 ± 3.32↑ | 6.07 ± 4.82 | 4.12 ± 1.40 | |
W-FKV | 7.61 ± 8.34 | 2.93 ± 2.19 | 3.46 ± 2.27 | 4.30 ± 3.22 | 5.89 ± 0.41↑ | 5.69 ± 1.15↑ | |
D21 | O-C | 1.16 ± 0.80 | 1.02 ± 0.22 | 1.04 ± 0.33 | 1.02 ± 0.25 | 1.21 ± 0.94 | 1.02 ± 0.25 |
O-HKV | 0.91 ± 0.42 | 1.44 ± 0.40 | 3.22 ± 0.44↑↑ | 0.70 ± 0.24 | 1.15 ± 0.32 | 2.12 ± 0.56↑ | |
O-FKV | 1.91 ± 0.26 | 0.90 ± 0.13 | 2.09 ± 0.45↑ | 1.60 ± 2.38 | 0.43 ± 0.52 | 2.20 ± 3.05 | |
W-C | 1.36 ±1.30 | 1.29 ± 0.93 | 1.17 ± 0.82 | 1.10 ± 0.55 | 1.70 ± 1.82 | 1.11 ± 0.65 | |
W-HKV | 1.25 ± 0.94 | 1.20 ± 1.30 | 2.01 ±1.45 | 1.82 ± 1.79 | 2.76 ± 2.03 | 2.23 ± 2.55 | |
W-FKV | 0.83 ±0.66 | 0.66 ± 0.53 | 0.93 ± 0.61 | 0.37 ± 0.10 | 0.79 ± 0.59 | 0.57 ± 0.19 |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
Submitted:
19 June 2023
Posted:
21 June 2023
Read the latest preprint version here
This version is not peer-reviewed
Submitted:
19 June 2023
Posted:
21 June 2023
Read the latest preprint version here
Treatment | Abbreviation | Number of fish Challenged | Number of Total Dead Fish at 15 dpc | RPS (%) 15 dpc |
---|---|---|---|---|
Water based (W) | ||||
Control (Cell culture supernatant, L15) | W-C | 31 | 5 | |
Heat-killed vaccine | W-HKV | 27 | 2 | 54.6 |
Formalin-killed vaccine | W-FKV | 31 | 2 | 59.7 |
Oil-based (O) (MontanideTM ISA 763A VG) | ||||
Control (L15 + Oil adjuvant) | O-C | 32 | 8 | |
Heat-killed vaccine | O-HKV | 28 | 5 | 32.0 |
Formalin-killed vaccine | O-FKV | 24 | 9 | 0 |
Gene | Oligo Sequences | Annealing Temperature (oC) | Product Size (bp) | Reference |
---|---|---|---|---|
EF-1α | F-5’-CTACAGCCAGGCTCGTTTCG-3’ R-5’-CTTGTCACTGGTCTCCAGCA-3’ |
56 | 139 | [30] |
IgM | F-5’-GGATGACGAGGAAGCAGACT-3’ R-5’-CATCATCCCTTTGCCACTGG-3’ |
53 | 122 | [30] |
IgT | F-5’-TGACCAGAAATGGCGAAGTCTG-3’ R-5’-GTTATAGTCACATTCTTTAGAATTACC-3’ |
53 | 163 | [30] |
IgD | F-5′- AACACCACCCTGTCCCTGAAT-3′ R-5’-GGGTGAAAACCACATTCCAAC-3’ |
61 | 127 | [31] |
Time Points | Treatments | Head Kidney | Spleen | ||||
---|---|---|---|---|---|---|---|
IgM | IgT | IgD | IgM | IgT | IgD | ||
D7 | O-C | 1.07 ± 0.46 | 1.02 ± 0.20 | 1.07 ± 0.44 | 1.51 ± 1.60 | 1.20 ± 0.75 | 1.05 ± 0.38 |
O-HKV | 1.75 ± 0.78 | 3.14 ± 0.73↑ | 2.93 ± 1.24↑ | 1.14 ± 0.45 | 0.90 ± 0.49 | 1.00 ± 0.78 | |
O-FKV | 1.53 ± 0.47 | 1.61 ± 0.99 | 1.89 ± 0.11↑ | 1.51 ± 0.94 | 0.93 ± 0.75 | 2.04 ± 1.34 | |
W-C | 1.55 ± 1.77 | 1.48 ± 1.61 | 1.07 ± 0.44 | 1.72 ± 1.97 | 1.79 ± 2.09 | 1.24 ± 0.86 | |
W-HKV | 0.96 ± 0.74 | 0.55 ± 0.32 | 1.19 ± 0.86 | 1.04 ±1.02 | 1.09 ± 0.96 | 1.23 ± 0.86 | |
W-FKV | 6.00 ± 2.81↑ | 1.43 ± 0.85 | 3.80 ± 1.81↑ | 0.31 ± 0.12 | 0.38 ± 0.22 | 0.42 ± 0.16 | |
D14 | O-C | 1.58 ± 1.21 | 1.09 ± 0.48 | 1.01 ± 0.17 | 1.19 ± 0.70 | 1.67 ± 1.72 | 1.20 ± 0.89 |
O-HKV | 4.94 ± 4.11 | 0.77 ± 0.45 | 1.46 ± 0.92 | 1.09 ± 0.80 | 1.00 ± 0.67 | 1.22 ± 1.04 | |
O-FKV | 1.84 ± 2.25 | 0.37 ± 0.36 | 1.08 ±1.14 | 1.81 ± 0.87 | 1.11 ± 0.32 | 2.71 ± 1.71 | |
W-C | 1.44 ± 1.05 | 0.96 ± 0.80 | 1.53 ± 1.70 | 1.43 ± 1.19 | 2.31 ± 2.80 | 1.66 ± 1.88 | |
W-HKV | 1.81 ± 0.64 | 1.09 ± 0.53 | 1.35 ± 0.45 | 6.91 ± 3.32↑ | 6.07 ± 4.82 | 4.12 ± 1.40 | |
W-FKV | 7.61 ± 8.34 | 2.93 ± 2.19 | 3.46 ± 2.27 | 4.30 ± 3.22 | 5.89 ± 0.41↑ | 5.69 ± 1.15↑ | |
D21 | O-C | 1.16 ± 0.80 | 1.02 ± 0.22 | 1.04 ± 0.33 | 1.02 ± 0.25 | 1.21 ± 0.94 | 1.02 ± 0.25 |
O-HKV | 0.91 ± 0.42 | 1.44 ± 0.40 | 3.22 ± 0.44↑↑ | 0.70 ± 0.24 | 1.15 ± 0.32 | 2.12 ± 0.56↑ | |
O-FKV | 1.91 ± 0.26 | 0.90 ± 0.13 | 2.09 ± 0.45↑ | 1.60 ± 2.38 | 0.43 ± 0.52 | 2.20 ± 3.05 | |
W-C | 1.36 ±1.30 | 1.29 ± 0.93 | 1.17 ± 0.82 | 1.10 ± 0.55 | 1.70 ± 1.82 | 1.11 ± 0.65 | |
W-HKV | 1.25 ± 0.94 | 1.20 ± 1.30 | 2.01 ±1.45 | 1.82 ± 1.79 | 2.76 ± 2.03 | 2.23 ± 2.55 | |
W-FKV | 0.83 ±0.66 | 0.66 ± 0.53 | 0.93 ± 0.61 | 0.37 ± 0.10 | 0.79 ± 0.59 | 0.57 ± 0.19 |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
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