Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

E7 Oncogene HPV 58 Variants Detected in Northeast Brazil: Genetic and Functional Analysis

Bárbara Simas Chagas
† ORCID logo ,
Elias Tibúrcio-Júnior
† ,
Ruany Cristyne de Oliveira Silva
,
Daffany Luana dos Santos
,
Marconi Rêgo Barros-Júnior
,
Rita de Cássia Pereira de Lima
,
Maria da Conceição Viana Invenção
ORCID logo ,
Pedro Luiz França Neto
,
Vanessa Emanuelle Pereira Santos
,
Antonio Hunberto Silva-Júnior
,
Jacinto Costa Silva-Neto
ORCID logo ,
Marcus Vinícius de Aragão Batista
,
Antonio Carlos de Freitas
* ORCID logo
†
These authors contributed equally to the work.
Version 1 : Received: 15 June 2023 / Approved: 16 June 2023 / Online: 16 June 2023 (10:01:35 CEST)

A peer-reviewed article of this Preprint also exists.

Chagas, B.S.; Tibúrcio Júnior, E.; Silva, R.C.O.; Santos, D.L.; Barros Junior, M.R.; Lima, R.C.P.; Invenção, M.C.V.; Santos, V.E.P.; França Neto, P.L.; Silva Júnior, A.H.; Silva Neto, J.C.; Batista, M.V.A.; Freitas, A.C. E7 Oncogene HPV58 Variants Detected in Northeast Brazil: Genetic and Functional Analysis. Microorganisms 2023, 11, 1915. Chagas, B.S.; Tibúrcio Júnior, E.; Silva, R.C.O.; Santos, D.L.; Barros Junior, M.R.; Lima, R.C.P.; Invenção, M.C.V.; Santos, V.E.P.; França Neto, P.L.; Silva Júnior, A.H.; Silva Neto, J.C.; Batista, M.V.A.; Freitas, A.C. E7 Oncogene HPV58 Variants Detected in Northeast Brazil: Genetic and Functional Analysis. Microorganisms 2023, 11, 1915.

Abstract

Cervical cancer is associated with persistent infections by high-risk HPV types that may have nucleotide polymorphisms and, consequently, different oncogenic potentials. Therefore, the objective of this study was to evaluate the genetic variability and structural effects of E7 oncogene of HPV58 in cervical scraping samples from Brazilian women. The study was carried out with patients from hospitals in metropolitan area of Recife, PE, Brazil. The most frequent HPV type was HPV16, 18 and 58, respectively. Phylogenetic analysis showed that the isolates were classified as sublineages A2, C1 and D2. Two positively selected mutations were found in E7: 63G and 64T. The mutations G41R, G63D and T64A in E7 protein were predicted to reduce the stability of the protein structure. Regarding the interaction of the E7 variant of HPV58 with the signaling of the NF-kB pathway, we observed that the variant HPV58/UFPE-54S decreased the activity of the pathway when compared to the prototype and the other variants behaved similarly to the prototype and the prototype increased the activity of the pathway when compared to pcDNA. In this study, it was possible to identify mutations that may interfere in the molecular interaction between the viral oncoproteins and host proteins.

Keywords

human papillomavirus 58; E7 oncogene; variants; phylogenetic analysis; gene expression

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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