Cheng, C.-Y.; Zhang, M.-Y.; Niu, Y.-C.; Zhang, M.; Geng, Y.-H.; Deng, H. Comparison of Fungal Genera Isolated from Cucumber Plants and Rhizosphere Soil by Using Various Cultural Media. J. Fungi2023, 9, 934.
Cheng, C.-Y.; Zhang, M.-Y.; Niu, Y.-C.; Zhang, M.; Geng, Y.-H.; Deng, H. Comparison of Fungal Genera Isolated from Cucumber Plants and Rhizosphere Soil by Using Various Cultural Media. J. Fungi 2023, 9, 934.
Cheng, C.-Y.; Zhang, M.-Y.; Niu, Y.-C.; Zhang, M.; Geng, Y.-H.; Deng, H. Comparison of Fungal Genera Isolated from Cucumber Plants and Rhizosphere Soil by Using Various Cultural Media. J. Fungi2023, 9, 934.
Cheng, C.-Y.; Zhang, M.-Y.; Niu, Y.-C.; Zhang, M.; Geng, Y.-H.; Deng, H. Comparison of Fungal Genera Isolated from Cucumber Plants and Rhizosphere Soil by Using Various Cultural Media. J. Fungi 2023, 9, 934.
Abstract
Plant endophytic fungi and rhizosphere soil fungi are often reported as biocontrol agents against plant pathogens or with plant growth promotion potential. Four treatments were performed in field and greenhouse experiments where cucumber plants were inoculated with Trichoderma harzianum and Fusarium oxysporum in 2022. The roots, stems and leaves of cucumber plants and their rhizosphere soil were collected twice individually from the field and greenhouse for isolation of cucumber endophytic and rhizosphere soil fungi. All 74 genera and 1275 fungal strains were sequenced by rDNA ITS. The PDA media yielded the highest number of genera isolated from cucumber plants, rhizosphere soil and both compared to other media. There were no significant differences among the 4 media for the isolation of cucumber endophytic fungi. The best media for isolating fungi from roots and stems were MRBA and PDA, respectively. PDA and CZA had higher isolation efficiency for the rhizosphere soil fungi than MRBA. The 28 fungal genera had high isolation efficiency, and the endophytic Trichoderma strains were best isolated by MEA. It is suggested that PDA can be used as a basic medium, and different media can be considered for specific fungal genera.
Keywords
plant endophytic fungi; rhizospheric soil fungi; isolation; cucumber; cultural media
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
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