Li, L.; Chang, Z.; Wei, K.; Tang, Y.; Chen, Z.; Zhang, H.; Wang, Y.; Zhu, H.; Feng, B. Chemical Differentiation and Quantitative Analysis of Black Ginseng Based on an LC-MS Combined with Multivariate Statistical Analysis Approach. Molecules2023, 28, 5251.
Li, L.; Chang, Z.; Wei, K.; Tang, Y.; Chen, Z.; Zhang, H.; Wang, Y.; Zhu, H.; Feng, B. Chemical Differentiation and Quantitative Analysis of Black Ginseng Based on an LC-MS Combined with Multivariate Statistical Analysis Approach. Molecules 2023, 28, 5251.
Li, L.; Chang, Z.; Wei, K.; Tang, Y.; Chen, Z.; Zhang, H.; Wang, Y.; Zhu, H.; Feng, B. Chemical Differentiation and Quantitative Analysis of Black Ginseng Based on an LC-MS Combined with Multivariate Statistical Analysis Approach. Molecules2023, 28, 5251.
Li, L.; Chang, Z.; Wei, K.; Tang, Y.; Chen, Z.; Zhang, H.; Wang, Y.; Zhu, H.; Feng, B. Chemical Differentiation and Quantitative Analysis of Black Ginseng Based on an LC-MS Combined with Multivariate Statistical Analysis Approach. Molecules 2023, 28, 5251.
Abstract
Black ginseng is a type of processed ginseng that is traditionally used as herbal medicine in East Asian countries. It is prepared from fresh, white, or red ginseng by undergoing a process of steaming and drying nine times. However, the chemical differentiation of black ginseng with different processing levels is not well understood. The aim of this study is to propose a new method for discriminating and quantifying black ginseng. Six ginsenosides from black ginseng were accurately quantified, and based on this, the black ginseng samples were divided into uncompleted and completed black ginseng. Ultra-high-performance liquid chromatography-quadrupole-time of flight/mass spectrometry (UPLC-Q-TOF/MS) combined with a multivariate statistical analysis strategy was then employed to differentiate the two groups. A total of 141 ions were selected as analytical markers of black ginseng, with 45 of these markers being annotated by matching precise m/z and MS/MS data from prior studies.
Keywords
Black ginseng; Ginsenosides; UPLC-Q-TOF/MS; processing; multivariate statistical analysis
Subject
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.