Version 1
: Received: 5 June 2023 / Approved: 8 June 2023 / Online: 8 June 2023 (14:02:33 CEST)
How to cite:
Zhu, W.; Wang, J.; Dong, Q.; Zhao, L.; Chen, J. Preferential Recruitment of Monocyte into the Brain is Orchestrated by VCAM-1 Expression. Preprints2023, 2023060649. https://doi.org/10.20944/preprints202306.0649.v1
Zhu, W.; Wang, J.; Dong, Q.; Zhao, L.; Chen, J. Preferential Recruitment of Monocyte into the Brain is Orchestrated by VCAM-1 Expression. Preprints 2023, 2023060649. https://doi.org/10.20944/preprints202306.0649.v1
Zhu, W.; Wang, J.; Dong, Q.; Zhao, L.; Chen, J. Preferential Recruitment of Monocyte into the Brain is Orchestrated by VCAM-1 Expression. Preprints2023, 2023060649. https://doi.org/10.20944/preprints202306.0649.v1
APA Style
Zhu, W., Wang, J., Dong, Q., Zhao, L., & Chen, J. (2023). Preferential Recruitment of Monocyte into the Brain is Orchestrated by VCAM-1 Expression. Preprints. https://doi.org/10.20944/preprints202306.0649.v1
Chicago/Turabian Style
Zhu, W., Lin Zhao and Jian Chen. 2023 "Preferential Recruitment of Monocyte into the Brain is Orchestrated by VCAM-1 Expression" Preprints. https://doi.org/10.20944/preprints202306.0649.v1
Abstract
The restriction of normal blood flow is the cause of many diseases including stroke and coronary artery diseases. To study the consequences of vessel blockade, previous models mainly focused on major arteries and have been well studied. However, the sequela from interruption of capillary vessels by microemboli was less well characterized. In this study, we exploited polystyrene microspheres as a mimicry of microemboli and found that microspheres of this size can be trapped in capillary vessels of all organs without causing apparent acute morbidities of the host. Interestingly, we accidentally found significantly increased recruitment of monocyte to the brain vasculature expressing low levels of Ly6C expression, but not to other organs. Further study revealed the spleen is the major origin of the recruited monocyte. Most importantly, VCAM-1 which is constitutively expressed on mouse brain vasculature orchestrates the recruitment of monocyte. Blockade of VCAM-1 in mice can substantially reduce monocyte recruitment. Interestingly, monocytes get activated through TNF- signaling which likely happens in the spleen instead of the brain. Collectively, we found a unique monocyte recruitment strategy in the brain comparing to other orangs, in response to capillary blockade induced by polystyrene microspheres.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
