Version 1
: Received: 7 June 2023 / Approved: 7 June 2023 / Online: 7 June 2023 (13:14:24 CEST)
How to cite:
Leonova, G.; Somova, L.; Abramva, S.; Pustovalov, E. Study of Viral Coinfection of the Ixodes Persulcatus Tickets Feeding on Humans in a Natural Foci of the South of the Far East. Preprints2023, 2023060557. https://doi.org/10.20944/preprints202306.0557.v1
Leonova, G.; Somova, L.; Abramva, S.; Pustovalov, E. Study of Viral Coinfection of the Ixodes Persulcatus Tickets Feeding on Humans in a Natural Foci of the South of the Far East. Preprints 2023, 2023060557. https://doi.org/10.20944/preprints202306.0557.v1
Leonova, G.; Somova, L.; Abramva, S.; Pustovalov, E. Study of Viral Coinfection of the Ixodes Persulcatus Tickets Feeding on Humans in a Natural Foci of the South of the Far East. Preprints2023, 2023060557. https://doi.org/10.20944/preprints202306.0557.v1
APA Style
Leonova, G., Somova, L., Abramva, S., & Pustovalov, E. (2023). Study of Viral Coinfection of the Ixodes Persulcatus Tickets Feeding on Humans in a Natural Foci of the South of the Far East. Preprints. https://doi.org/10.20944/preprints202306.0557.v1
Chicago/Turabian Style
Leonova, G., Svetlana Abramva and Evgeniy Pustovalov. 2023 "Study of Viral Coinfection of the Ixodes Persulcatus Tickets Feeding on Humans in a Natural Foci of the South of the Far East" Preprints. https://doi.org/10.20944/preprints202306.0557.v1
Abstract
The phenomenon of pathogens co-infection detected in the half fed on humans I. persulcatus tick in the south of the Far East was studied. Researchs were carried out on PEK, Vero, Vero-E6 cell lines, outbred mice, chicken embryos, using ELISA, PCR, IMFA, plaque formation, and electron microscopy. The tick contained an antigen and a genetic marker of the tick-borne encephalitis virus (TBEV). The patient had post-vaccination antibodies in a titer of 1:200, as a result of which, obviously, an antibody-dependent elimination of TBEV occurred. The tick-borne co-isolate also contained an unknown pathogen (Kiparisovo-144 virus), which, in our opinion, was a trigger for the activation of chronic infection in suckling white mice. In the laboratory coisolate, ectromelia virus was present, as evidenced by paw edema during intradermal infection of mice, characteristic rashes on the chorion-allantoic envilope of chicken embryos, and typical plaques on Vero-E6. The Kiparisovo-144 virus was not pathogenic for white mice and chicken embryos, but successfully multiplied in the PEK, Vero, and Vero-E6 lines. Viral co-infection was confirmed by electron microscopy. Passaging on mice contributed to an increase in the virulence of the co-isolate, whose titer increased by 10,000 times by the 5th passage, which poses a serious epidemiological danger.
Keywords
coinfection; tick Ixodes persulcatus; virus isolate; tick-borne encephalitis virus (TBEV); ectromelia virus; biological properties of co-isolate; electron microscopy; south of the Russian Far East
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
