Circulating miRNA As Prognostic Biomarker During Estrus of Buffalo

How to cite: Murikipudi, N.S.; Singh, P.; Yadav, S.K.; Singh, R.; Singh, C.; Kumar, P.; Bhardwaj, A.; Kumar, D.; Kumar, R.; Selokar, N.; Kumar, S.; Saxena, N.; Sharma, R.K.; Onteru, S.K.; Singh, D.; Bharadwaj, A.; Kumar, R.; Dutta, T.K.; Nayan, V. Circulating miRNA As Prognostic Biomarker During Estrus of Buffalo. Preprints 2023, 2023060370. https://doi.org/10.20944/preprints202306.0370.v1 Murikipudi, N.S.; Singh, P.; Yadav, S.K.; Singh, R.; Singh, C.; Kumar, P.; Bhardwaj, A.; Kumar, D.; Kumar, R.; Selokar, N.; Kumar, S.; Saxena, N.; Sharma, R.K.; Onteru, S.K.; Singh, D.; Bharadwaj, A.; Kumar, R.; Dutta, T.K.; Nayan, V. Circulating miRNA As Prognostic Biomarker During Estrus of Buffalo. Preprints 2023, 2023060370. https://doi.org/10.20944/preprints202306.0370.v1

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MDPI and ACS Style

Murikipudi, N.S.; Singh, P.; Yadav, S.K.; Singh, R.; Singh, C.; Kumar, P.; Bhardwaj, A.; Kumar, D.; Kumar, R.; Selokar, N.; Kumar, S.; Saxena, N.; Sharma, R.K.; Onteru, S.K.; Singh, D.; Bharadwaj, A.; Kumar, R.; Dutta, T.K.; Nayan, V. Circulating miRNA As Prognostic Biomarker During Estrus of Buffalo. Preprints 2023, 2023060370. https://doi.org/10.20944/preprints202306.0370.v1

APA Style

Murikipudi, N.S., Singh, P., Yadav, S.K., Singh, R., Singh, C., Kumar, P., Bhardwaj, A., Kumar, D., Kumar, R., Selokar, N., Kumar, S., Saxena, N., Sharma, R.K., Onteru, S.K., Singh, D., Bharadwaj, A., Kumar, R., Dutta, T.K., & Nayan, V. (2023). Circulating miRNA As Prognostic Biomarker During Estrus of Buffalo. Preprints. https://doi.org/10.20944/preprints202306.0370.v1

Chicago/Turabian Style

Murikipudi, N.S., Tirtha Kumar Dutta and Varij Nayan. 2023 "Circulating miRNA As Prognostic Biomarker During Estrus of Buffalo" Preprints. https://doi.org/10.20944/preprints202306.0370.v1

Abstract

Buffalo are silent breeders, therefore detecting estrus is a serious challenge. There is a rising need for sensitive and precise biomarkers in this scenario. Recent research on miRNA has demon-strated the importance of these molecules as biomarkers. Though there have been miRNA studies in saliva during the estrous cycle, there have been few miRNA research in blood samples. The current study was designed to look at blood miRNAs during the oestrous cycle in heifers (n=5) to address the issue of silent estrus. On the day of estrus and diestrus, blood samples from 60 heifers were obtained and pooled into (n=5) separate samples. Ultrasonography and progesterone assay was performed to confirm estrus. Then, employing particular miRNA adapters, small RNA se-quencing of miRNA was performed using the Illumina Miseq 2500. The UEA sRNA bioinfor-matics workbench identified 94 substantially differently expressed miRNAs (p>0.05) from these data. In estrus, 63 miRNA were upregulated and 31 miRNA were downregulated. When fold change was increased to (log2foldchange >1; q value less than 0.05), 25 miRNAs were elevated during estrus. miR-497, miR-582, miR-10174, miR-23, miR-223, miR-1296 were upregulated, whereas miR-10167, 671, 1246,122 were downregulated. miR-497 is unusually elevated (log2 foldchange>5) when compared to another miRNA (log2 foldchange >5) miRNet 2.0, Cytoscape, and MIENTURNET network software found that miR-497 has more degree centrality, above 60; it is associated with more than 60 nodes, followed by miR-93

Keywords

miRNA; Buffalo; Estrus; Prognostic biomarker; Gene-Target prediction

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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