Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Budesonide Analogues Preserve Stem Cell Pluripotency and Delay 3D Gastruloid Development

Version 1 : Received: 30 May 2023 / Approved: 30 May 2023 / Online: 30 May 2023 (13:48:16 CEST)

A peer-reviewed article of this Preprint also exists.

Amoroso, F.; Ibello, E.; Saracino, F.; Cermola, F.; Ponticelli, G.; Scalera, E.; Ricci, F.; Villetti, G.; Cobellis, G.; Minchiotti, G.; Patriarca, E.J.; De Cesare, D.; D’Aniello, C. Budesonide Analogues Preserve Stem Cell Pluripotency and Delay 3D Gastruloid Development. Pharmaceutics 2023, 15, 1897. Amoroso, F.; Ibello, E.; Saracino, F.; Cermola, F.; Ponticelli, G.; Scalera, E.; Ricci, F.; Villetti, G.; Cobellis, G.; Minchiotti, G.; Patriarca, E.J.; De Cesare, D.; D’Aniello, C. Budesonide Analogues Preserve Stem Cell Pluripotency and Delay 3D Gastruloid Development. Pharmaceutics 2023, 15, 1897.

Abstract

Small molecules that can modulate or stabilize cell-cell interactions are valuable tools for investigating the impact of collective cell behavior on various biological processes such as development/morphogenesis, tissue regeneration and cancer progression. Recently, we showed that budesonide, a glucocorticoid widely used as anti-asthmatic drug, is a potent regulator of stem cell pluripotency. Here we tested the effect of different budesonide derivatives and identified CHD-030498 as a more effective analogue of budesonide. CHD-030498 was able to prevent stem cell pluripotency exit in different cell-based models, including embryonic stem-to-mesenchymal transition, spontaneous differentiation and 3D gastruloid development, and at lower doses compared to budesonide.

Keywords

drug toxicity; budesonide analogues; stem cells; pluripotency exit; 3D gastruloids

Subject

Biology and Life Sciences, Cell and Developmental Biology

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