Version 1
: Received: 26 May 2023 / Approved: 30 May 2023 / Online: 30 May 2023 (04:20:02 CEST)
How to cite:
Araujo, G.S.D.; Ferreira, A.L.G.; Soares, A.M.V.D.M.; Abessa, D.M.D.S.; Loureiro, S. Multi-Generation Effects of Lead (Pb) on Two Daphnia Species: Looking at Different Levels of Biological Organization. Preprints2023, 2023052036. https://doi.org/10.20944/preprints202305.2036.v1
Araujo, G.S.D.; Ferreira, A.L.G.; Soares, A.M.V.D.M.; Abessa, D.M.D.S.; Loureiro, S. Multi-Generation Effects of Lead (Pb) on Two Daphnia Species: Looking at Different Levels of Biological Organization. Preprints 2023, 2023052036. https://doi.org/10.20944/preprints202305.2036.v1
Araujo, G.S.D.; Ferreira, A.L.G.; Soares, A.M.V.D.M.; Abessa, D.M.D.S.; Loureiro, S. Multi-Generation Effects of Lead (Pb) on Two Daphnia Species: Looking at Different Levels of Biological Organization. Preprints2023, 2023052036. https://doi.org/10.20944/preprints202305.2036.v1
APA Style
Araujo, G.S.D., Ferreira, A.L.G., Soares, A.M.V.D.M., Abessa, D.M.D.S., & Loureiro, S. (2023). Multi-Generation Effects of Lead (Pb) on Two Daphnia Species: Looking at Different Levels of Biological Organization. Preprints. https://doi.org/10.20944/preprints202305.2036.v1
Chicago/Turabian Style
Araujo, G.S.D., Denis Moledo de Souza Abessa and Susana Loureiro. 2023 "Multi-Generation Effects of Lead (Pb) on Two Daphnia Species: Looking at Different Levels of Biological Organization" Preprints. https://doi.org/10.20944/preprints202305.2036.v1
Abstract
Two monophyletic Daphnia species (Daphnia magna and D. similis) were exposed to a sub-lethal concentration of Pb (50 µg/L) for nine generations under two food regimes (usual and restricted) and analysed for acetylcholinesterase (AChE) activity, hatching delay and lifespan, and Net Reproductive Rate (R0) at subcellular, individual and population levels, respectively. At the sixth generation, Pb acclimated neonates were moved to a clean media for three more generations to check for recovery. D. magna showed no Pb effect on Net Reproductive Rate (R0). However, Pb stimulated reproduction, reduced lifespan and decreased AChE activity. Hatching delay and lifespan did not improve during the recovery process, indicating genetic adaptation. Food restriction reduced R0, lifespan, delayed hatching, and increased AChE activity; opposite outcomes were shown for D. similis. Full recovery shown by R0 suggests a physiological acclimation of D. similis. Under food restriction, the animals exhibited a reduction of R0 and lifespan, delayed hatching and increased AChE activity; however, with no Pb effect. The recovery process under food restriction showed that D. similis might not cope with Pb exposure, indicating failed recovery. Such outcomes indicate that a model species' sensitivity may not represent another's sensitivity.
Keywords
Multi-generation; Net Reproductive Rate (R0); Hatching delay; lifespan; acetylcholinesterase
Subject
Environmental and Earth Sciences, Pollution
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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