Huang, L.-H.; Rau, C.-S.; Liu, Y.-W.; Wu, C.-J.; Chien, P.-C.; Lin, H.-P.; Wu, Y.-C.; Huang, C.-Y.; Hsieh, T.-M.; Hsieh, C.-H. XIST-microRNAs/mRNA Network in Circulating CD4+ T Cells of the Patients with Hepatocellular Carcinoma. Preprints2023, 2023051976. https://doi.org/10.20944/preprints202305.1976.v1
APA Style
Huang, L. H., Rau, C. S., Liu, Y. W., Wu, C. J., Chien, P. C., Lin, H. P., Wu, Y. C., Huang, C. Y., Hsieh, T. M., & Hsieh, C. H. (2023). XIST-microRNAs/mRNA Network in Circulating CD4<sup>+</sup> T Cells of the Patients with Hepatocellular Carcinoma. Preprints. https://doi.org/10.20944/preprints202305.1976.v1
Chicago/Turabian Style
Huang, L., Ting-Min Hsieh and Ching-Hua Hsieh. 2023 "XIST-microRNAs/mRNA Network in Circulating CD4<sup>+</sup> T Cells of the Patients with Hepatocellular Carcinoma" Preprints. https://doi.org/10.20944/preprints202305.1976.v1
Abstract
Hepatocellular carcinoma (HCC) is one of the most common cancers and the main cause of cancer-related death globally. Immune dysregulation of CD4+ T cells has been identified as a role in the development of HCC. Nevertheless, the underlying molecular pathways of CD4+ T cells in HCC are not completely known. Thus, a better understanding of the dysregulation of lncRNA-miRNA/mRNA network might yield novel insights into the etiology or progression of HCC. In this study, circulating CD4+ T cells were isolated from the whole blood of 10 healthy controls and 10 HCC patients for next-generation sequencing of the expression of lncRNAs, miRNAs, and mRNAs. Our data showed that there was different expression of 34 transcripts (two lncRNAs, XIST and MIR222HG, 29 mRNA, and 3 other types of RNA) and 13 miRNAs in the circulating CD4+ T cells of HCC patients. The expression of the lncRNA XIST-related miRNAs and their target mRNAs was confirmed using real-time quantitative polymerase chain reaction (qPCR) on samples from 100 healthy controls and 60 HCC patients. The lncRNA–miRNA/mRNA regulation network was created using interaction data generated from ENCORI and revealed there are positive correlations in the infiltration of total CD4+ T cells and negative correlations in the infiltration of Th1 CD4+ T cells.
Keywords
hepatocellular carcinoma (HCC); CD4+ T cells; next generation sequencing (NGS); long non-coding RNAs (lncRNAs); microRNAs (miRNAs)
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
