Version 1
: Received: 12 May 2023 / Approved: 15 May 2023 / Online: 15 May 2023 (12:43:54 CEST)
How to cite:
Ivashchenko, A.; Rakhmetullina, A.; Akimniyazova, A.; Niyazova, T.; Pyrkova, A.; Tauassarova, M.; Zielenkiewicz, P. Extraordinary Interactions of piRNAs with mRNA of Candidate Genes in Esophageal Squamous Cell Carcinoma. Preprints2023, 2023051046. https://doi.org/10.20944/preprints202305.1046.v1
Ivashchenko, A.; Rakhmetullina, A.; Akimniyazova, A.; Niyazova, T.; Pyrkova, A.; Tauassarova, M.; Zielenkiewicz, P. Extraordinary Interactions of piRNAs with mRNA of Candidate Genes in Esophageal Squamous Cell Carcinoma. Preprints 2023, 2023051046. https://doi.org/10.20944/preprints202305.1046.v1
Ivashchenko, A.; Rakhmetullina, A.; Akimniyazova, A.; Niyazova, T.; Pyrkova, A.; Tauassarova, M.; Zielenkiewicz, P. Extraordinary Interactions of piRNAs with mRNA of Candidate Genes in Esophageal Squamous Cell Carcinoma. Preprints2023, 2023051046. https://doi.org/10.20944/preprints202305.1046.v1
APA Style
Ivashchenko, A., Rakhmetullina, A., Akimniyazova, A., Niyazova, T., Pyrkova, A., Tauassarova, M., & Zielenkiewicz, P. (2023). Extraordinary Interactions of piRNAs with mRNA of Candidate Genes in Esophageal Squamous Cell Carcinoma. Preprints. https://doi.org/10.20944/preprints202305.1046.v1
Chicago/Turabian Style
Ivashchenko, A., Makpal Tauassarova and Piotr Zielenkiewicz. 2023 "Extraordinary Interactions of piRNAs with mRNA of Candidate Genes in Esophageal Squamous Cell Carcinoma" Preprints. https://doi.org/10.20944/preprints202305.1046.v1
Abstract
Recently, a database of human piRNAs has been created, which allows studying the binding of many piRNAs to mRNAs of genes involved in many diseases, including cancer. In the present work, we investigated what piRNAs can interact with candidate esophageal squamous cell carcinoma (ESCC) genes. The binding of 480 thousand piRNAs with mRNAs of 66 candidate ESCC genes was studied. Bioinformatic studies found that piRNAs bind only to the mRNAs of nine candidate genes: AURKA, BMP7, GCOM1, ERCC1, MTHFR, SASH1, SIX4, SULT1A1, and TP53. It has been shown that piRNAs can bind to mRNA by overlapping nucleotide sequences in limited 3'UTR and 5'UTR regions called clusters of binding sites (BSs). The existence of clusters of piRNA BSs significantly reduces the proportion of nucleotide sequences of these sites in the mRNA of target genes. Competition between piRNAs occurs for binding to the mRNA of target genes. Individual piRNAs and groups of piRNAs that have separate BSs and clusters of BSs in the mRNAs of two or more candidate genes have been identified in the mRNAs of these genes. This organization of piRNAs BSs indicates the interdependence of the expression of candidate genes through piRNAs. Significant differences in the ability of genes to interact with piRNAs avoid the side effects of piRNAs on genes with the lack of binding of such piRNAs. Individual piRNAs and sets of piRNAs are proposed and recommended for the diagnosis and therapy of ESCC.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.