Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Long-Chain Acyl Coenzyme a Dehydrogenase, a Key Player in Metabolic Rewiring/Invasiveness in Experimental Tumors and Human Mesothelioma Cell Lines

Daniel L. Pouliquen
* ORCID logo ,
Giacomo Ortone
,
Letizia Rumiano
,
Alice Boissard
,
Cécile Henry
,
Stéphanie Blandin
ORCID logo ,
Catherine Guette
,
Chiara Riganti
ORCID logo ,
Joanna Kopecka
ORCID logo
Version 1 : Received: 12 May 2023 / Approved: 15 May 2023 / Online: 15 May 2023 (05:27:55 CEST)

A peer-reviewed article of this Preprint also exists.

Pouliquen, D.L.; Ortone, G.; Rumiano, L.; Boissard, A.; Henry, C.; Blandin, S.; Guette, C.; Riganti, C.; Kopecka, J. Long-Chain Acyl Coenzyme A Dehydrogenase, a Key Player in Metabolic Rewiring/Invasiveness in Experimental Tumors and Human Mesothelioma Cell Lines. Cancers 2023, 15, 3044. Pouliquen, D.L.; Ortone, G.; Rumiano, L.; Boissard, A.; Henry, C.; Blandin, S.; Guette, C.; Riganti, C.; Kopecka, J. Long-Chain Acyl Coenzyme A Dehydrogenase, a Key Player in Metabolic Rewiring/Invasiveness in Experimental Tumors and Human Mesothelioma Cell Lines. Cancers 2023, 15, 3044.

Abstract

Cross-species investigations on cancer invasiveness are a new approach that identified new biomarkers potentially useful for improving tumor diagnosis and prognosis in clinical medicine and veterinary science. In this study, we combined proteomic analysis of four experimental rat malignant mesothelioma (MM) tumors with analysis of ten patient-derived cell lines to identify common features associated to mitochondrial proteome rewiring. The comparison of significant abundance changes between invasive and non-invasive rat tumors rat tumors gave a list of 433 proteins, including twenty-six proteins reported to be exclusively located in mitochondria. Next, we analyzed the differential expression of genes encoding the mitochondrial proteins of interest in five primary epithelioid and five primary sarcomatoid human MM cell lines, and the most impressive increase was observed in the expression of the long-chain acyl coenzyme A dehydrogenase (ACADL). To evaluate the role of this enzyme in the migration/invasiveness, two epithelioid and two sarcomatoid human MM cell lines derived from patients with the highest and lowest overall survival were studied. Interestingly, sarcomatoid vs epithelioid cell lines were characterized by higher migration and fatty oxidation rates, in agreement with ACADL findings. These results suggest that evaluating mitochondrial proteins in MM specimens might identify tumors with higher invasiveness.

Keywords

malignant mesothelioma; metabolism; mitochondria; long-chain specific acyl-CoA dehydrogenase; fatty acid β-oxidation, biomarker

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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