Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effects of UHDR and Conventional Irradiation on Behavioral and Cognitive Performance and the Percent Ly6G+ CD45+ Cells in the Hippocampus

Ariel Chaklai
,
Pamela Canaday
,
Abigail O'Niel
,
Francis A. Cucinotta
,
Austin Sloop
,
David Gladstone
,
Brian Pogue
,
Rongxiao Zhang
,
Jacob Sunnerberg
,
Alireza Kheirolla
,
Charles R. Thomas
,
P. Jack Hoopes
,
Jacob Raber
* ORCID logo
Version 1 : Received: 8 May 2023 / Approved: 10 May 2023 / Online: 10 May 2023 (03:00:53 CEST)

How to cite: Chaklai, A.; Canaday, P.; O'Niel, A.; Cucinotta, F.A.; Sloop, A.; Gladstone, D.; Pogue, B.; Zhang, R.; Sunnerberg, J.; Kheirolla, A.; Thomas, C.R.; Hoopes, P.J.; Raber, J. Effects of UHDR and Conventional Irradiation on Behavioral and Cognitive Performance and the Percent Ly6G+ CD45+ Cells in the Hippocampus. Preprints 2023, 2023050673. https://doi.org/10.20944/preprints202305.0673.v1 Chaklai, A.; Canaday, P.; O'Niel, A.; Cucinotta, F.A.; Sloop, A.; Gladstone, D.; Pogue, B.; Zhang, R.; Sunnerberg, J.; Kheirolla, A.; Thomas, C.R.; Hoopes, P.J.; Raber, J. Effects of UHDR and Conventional Irradiation on Behavioral and Cognitive Performance and the Percent Ly6G+ CD45+ Cells in the Hippocampus. Preprints 2023, 2023050673. https://doi.org/10.20944/preprints202305.0673.v1

Abstract

In the current study, we assessed the effects of conventional and Ultra High Dose Rate (UHDR) irradiation on behavioral and cognitive performance one month following exposure and assessed whether these effects were associated with alterations in the number of immune cells in the hippocampus using flow cytometry. Conventional- and UHDR-irradiated mice showed impaired novel object recognition. During fear learning, conventional- and UHDR-irradiated mice moved less during the inter-stimulus-interval (ISI) and UHDR-irradiated mice also moved less during the baseline period (prior to the first tone). In irradiated mice, reduced activity levels were also seen in the home cage; conventional- and UHDR-irradiated mice moved less during the light period and UHDR-irradiated mice moved less during the dark period. Following behavioral and cognitive testing, infiltrating immune cells in the hippocampus were analyzed by flow cytometry. The percent Ly6G+ CD45+ cells in the hippocampus was lower in conventional- and UHDR-irradiated than sham-irradiated mice, suggesting that neutrophils might be particularly sensitive to radiation. The percent of Ly6G+ CD45+ cells in the hippocampus was positively correlated with the time spent exploring the novel object in the object recognition test, suggesting that the reduced percent Ly6G+ CD45+ cells in the hippocampus might mediate some of the detrimental radiation-induced cognitive effects.

Keywords

FLASH; UHDR; hippocampus; behavioral performance; cognitive performance; flow cytometry

Subject

Biology and Life Sciences, Neuroscience and Neurology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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