Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Molecular Detection and Phylogenetic Analyses of Babesia spp. and Theileria spp. In Livestock in Bangladesh

Uday Kumar Mohanta
,
Boniface Chikufenji
ORCID logo ,
Eloiza May Galon
ORCID logo ,
Shengwei Ji
,
Zhuowei Ma
,
Shimaa Abd El‒Salam El‒Sayed
ORCID logo ,
Aaron Edmond Ringo
,
Thanh Thom Do
,
Xuenan Xuan
* ORCID logo
Version 1 : Received: 27 April 2023 / Approved: 28 April 2023 / Online: 28 April 2023 (04:19:13 CEST)

A peer-reviewed article of this Preprint also exists.

Mohanta, U.K.; Chikufenji, B.; Galon, E.M.; Ji, S.; Ma, Z.; El-Sayed, S.A.-S.; Ringo, A.E.; Do, T.T.; Xuan, X. Molecular Detection and Phylogenetic Analyses of Babesia spp. and Theileria spp. in Livestock in Bangladesh. Microorganisms 2023, 11, 1563. Mohanta, U.K.; Chikufenji, B.; Galon, E.M.; Ji, S.; Ma, Z.; El-Sayed, S.A.-S.; Ringo, A.E.; Do, T.T.; Xuan, X. Molecular Detection and Phylogenetic Analyses of Babesia spp. and Theileria spp. in Livestock in Bangladesh. Microorganisms 2023, 11, 1563.

Abstract

Piroplasmosis, caused by Babesia spp. and Theileria spp., poses significant constraints for livestock production and upgradation in Bangladesh. Besides examining blood smears, few molecular reports are available from some selected areas in the country. Therefore, the actual scenario of piroplasmosis in Bangladesh is deficient. This study aimed to screen the piroplasms in different livestock species by molecular tools. A total of 276 blood samples were collected from cattle, gayals (Bos frontalis) and goats in five geographies of Bangladesh. Thereafter, screening was conducted through a polymerase chain reaction, and species were confirmed by sequencing. The prevalence of Babesia bigemina, B. bovis, B. naoakii, B. ovis, Theileria annulata and T. orientalis was 49.28%, 0.72%, 1.09%, 32.26%, 6.52% and 46.01%, respectively. The highest prevalence (79/109; 72.48%) of co-infections was observed with B. bigemina and T. orientalis. The phylogenetic analyses revealed that the sequences of B. bigemina, B. bovis, B. naoakii, B. ovis and T. annulata were included in one clade in the respective phylogram. In contrast, the sequences of T. orientalis were separated into two clades, corresponding to Type 5 and 7. To the best of our knowledge, this is the first molecular report on piroplasms in gayals and goats in Bangladesh.

Keywords

Babesia spp.; Theileria spp.; molecular detection; phylogeny; Livestock; Bangladesh

Subject

Biology and Life Sciences, Parasitology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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