Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

The Influence of Glycerol Flow through a Shielded Coil on an Enzyme Placed Near Its Side

Yuri D. Ivanov
* ORCID logo ,
Ivan D. Shumov
,
Andrey F. Kozlov
,
Maria O. Ershova
,
Anastasia A. Valueva
,
Irina A. Ivanova
,
Vadim Y. Tatur
,
Andrei A. Lukyanitsa
,
Nina D. Ivanova
,
Vadim S. Ziborov
Version 1 : Received: 23 April 2023 / Approved: 24 April 2023 / Online: 24 April 2023 (06:14:42 CEST)
Version 2 : Received: 10 June 2023 / Approved: 12 June 2023 / Online: 12 June 2023 (04:15:30 CEST)

A peer-reviewed article of this Preprint also exists.

Ivanov, Y.D.; Shumov, I.D.; Kozlov, A.F.; Ershova, M.O.; Valueva, A.A.; Ivanova, I.A.; Tatur, V.Y.; Lukyanitsa, A.A.; Ivanova, N.D.; Ziborov, V.S. Glycerol Flow through a Shielded Coil Induces Aggregation and Activity Enhancement of Horseradish Peroxidase. Appl. Sci. 2023, 13, 7516. Ivanov, Y.D.; Shumov, I.D.; Kozlov, A.F.; Ershova, M.O.; Valueva, A.A.; Ivanova, I.A.; Tatur, V.Y.; Lukyanitsa, A.A.; Ivanova, N.D.; Ziborov, V.S. Glycerol Flow through a Shielded Coil Induces Aggregation and Activity Enhancement of Horseradish Peroxidase. Appl. Sci. 2023, 13, 7516.

Abstract

Glycerol has found its applications as a heat-transfer fluid in heat exchangers, and as a compo-nent of functional solutions in biosensor analysis. Flowing non-aqueous fluids are known to be able to induce electromagnetic fields due to triboelectric effect. These triboelectrically generated electromagnetic fields can affect biological macromolecules. Horseradish peroxidase (HRP) is widely employed as a convenient model object for studying how external electric, magnetic, and electromagnetic fields affect enzymes. Herein, we have studied whether the flow of glycerol in a ground-shielded cylindrical coil affects the HRP enzyme incubated at a 2 cm distance near the coil’s side. Atomic force microscopy (AFM) has been employed in order to study the effect of glycerol flow on HRP at the nanoscale. Increased aggregation of HRP on mica has been observed after the incubation of the enzyme near the coil. Moreover, the enzymatic activity of HRP has also been affected. The results reported can find their application in biotechnology, food technology and life sciences application, considering the development of triboelectric generators, en-zyme-based biosensors and bioreactors with surface-immobilized enzymes. Our data can also be of interest for scientists studying triboelectric phenomena.

Keywords

atomic force microscopy; horseradish peroxidase; enzyme aggregation; enzymatic activity; tribo-electric effect; fluid flow

Subject

Biology and Life Sciences, Biophysics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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