Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Circulating CD34+/CD38-/CD26+ Leukemia Stem Cells along Chronic Myeloid Leukemia progression: differences between Chronic, Accelerated and Blast Phase

Paola Pacelli
* ,
Anna Sicuranza
* ORCID logo ,
Elena Bestoso
,
Alessandra Iurlo
ORCID logo ,
Elisabetta Abruzzese
ORCID logo ,
Adele Santoni
,
Margherita Malchiodi
,
Cristina Marzano
,
Elisabetta Zappone
,
Donatella Raspadori
ORCID logo ,
Monica Bocchia
ORCID logo
Version 1 : Received: 19 April 2023 / Approved: 20 April 2023 / Online: 20 April 2023 (07:31:53 CEST)

How to cite: Pacelli, P.; Sicuranza, A.; Bestoso, E.; Iurlo, A.; Abruzzese, E.; Santoni, A.; Malchiodi, M.; Marzano, C.; Zappone, E.; Raspadori, D.; Bocchia, M. Circulating CD34+/CD38-/CD26+ Leukemia Stem Cells along Chronic Myeloid Leukemia progression: differences between Chronic, Accelerated and Blast Phase. Preprints 2023, 2023040616. https://doi.org/10.20944/preprints202304.0616.v1 Pacelli, P.; Sicuranza, A.; Bestoso, E.; Iurlo, A.; Abruzzese, E.; Santoni, A.; Malchiodi, M.; Marzano, C.; Zappone, E.; Raspadori, D.; Bocchia, M. Circulating CD34+/CD38-/CD26+ Leukemia Stem Cells along Chronic Myeloid Leukemia progression: differences between Chronic, Accelerated and Blast Phase. Preprints 2023, 2023040616. https://doi.org/10.20944/preprints202304.0616.v1

Abstract

In Chronic Myeloid Leukemia (CML) patients, CD34+/CD38-/CD26+ cell population represents a “CML specific” Leukemia Stem Cell (LSC) compartment. Indeed, preliminary studies showed that the expression of CD26 discriminates bone marrow CML Leukemic Stem Cells (LSCs) from nor-mal Hematopoietic Stem Cells (HSCs) or from LSCs of other myeloid neoplasms. We were first to demonstrate that at diagnosis CD34+/CD38-/CD26+ cells are easily measurable also in Peripheral Blood (PB) and that residual circulating CD26+LSCs persist, with a fluctuating trend, in most pa-tients in optimal response during treatment with Tyrosine Kinase Inhibitors (TKIs) and even after successful TKI discontinuation. These data corroborate and confirm the possibility of using flow-cytometry CD26+ evaluation as an important diagnostic tool that, combined with molecular biology and cytogenetic, could provide a rapid diagnosis of Chronic Phase (CP) CML starting from a simple PB sample. Yet, few data are available regarding the behavior of CD26+LSCs during Accelerated Phase (AP) or Blast Phase (BP) CML and the role, if any, this peculiar staminal cell compartment may play in disease progression. In the present study we compared the presence and phenotypic characteristics of circulating CD26+LSCs in CP CML patients at diagnosis, during AP and in cases of progression to lymphoid BP, inquiring a possible role of these cells during dis-ease evolution.

Keywords

CP-CML; Accelerated Phase (AP); Blast Phase (BP); CD26+LSCs

Subject

Medicine and Pharmacology, Hematology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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