Preprint Article Version 3 Preserved in Portico This version is not peer-reviewed

Detection of Mutations and Genotyping of Drug-Resistant Mycobacterium tuberculosis Strains Isolated from Patients in Rural Eastern Cape Province

Lindiwe Modest Faye
* ORCID logo ,
Robin Warren
,
Sandeep Vasaikar
ORCID logo ,
Mojisola C. Hosu
,
Ncomeka Sineke
,
Selien Oostvogels
,
Anzaan Dippenaar
,
Teke Apalata
Version 1 : Received: 5 April 2023 / Approved: 6 April 2023 / Online: 6 April 2023 (09:24:17 CEST)
Version 2 : Received: 4 May 2023 / Approved: 5 May 2023 / Online: 5 May 2023 (07:26:31 CEST)
Version 3 : Received: 8 June 2023 / Approved: 9 June 2023 / Online: 9 June 2023 (15:36:21 CEST)

A peer-reviewed article of this Preprint also exists.

Faye, L.M.; Hosu, M.C.; Oostvogels, S.; Dippenaar, A.; Warren, R.M.; Sineke, N.; Vasaikar, S.; Apalata, T. The Detection of Mutations and Genotyping of Drug-Resistant Mycobacterium tuberculosis Strains Isolated from Patients in the Rural Eastern Cape Province. Infect. Dis. Rep. 2023, 15, 403-416. Faye, L.M.; Hosu, M.C.; Oostvogels, S.; Dippenaar, A.; Warren, R.M.; Sineke, N.; Vasaikar, S.; Apalata, T. The Detection of Mutations and Genotyping of Drug-Resistant Mycobacterium tuberculosis Strains Isolated from Patients in the Rural Eastern Cape Province. Infect. Dis. Rep. 2023, 15, 403-416.

Abstract

Drug-resistant tuberculosis (DR-TB) is still a major public health concern in South Africa. Mutations in M. tuberculosis can cause varying levels of phenotypic resistance to anti-TB medications. There have been no prior studies on gene mutations and the genotyping of DR-TB in the rural Eastern Cape Province; hence we aimed to identify DR-TB mutations, genetic diversity and allocated lineages among patients in this area. Using Xpert® MTB/RIF, we assessed the rifampin-resistance of sputum samples collected from 1157 patients suspected of having tuberculosis. GenoType MTBDR plus VER 2.0 was used for the detection of mutations causing resistance to anti-TB medications. The next step was to spotlight type 441 isolates. The most prevalent rifampin resistance-conferring mutations were in rpoB codon S531L, in INH-resistant strains, katG gene at codon S315TB and the inhA gene at codon C-15TB had highest mutations; 54.5% and 24.7%, respectively. In addition, 24.6% of strains showed mutations in both the rpoB and inhA genes, while 69.9% of strains showed mutations in both the katG and rpoB genes. Heteroresistance was seen in 17.9% of all cases in the study. According to spoligotyping analysis, Beijing families predominated. Investigating the evolutionary lineages of M. tuberculosis isolates can be done using the information provided by the study's diversity of mutations. In locations where these mutations have been discovered, decision-making regarding standardization of treatment regimens or individualized treatment may be aided by the detection frequency of rpoB, katG, and inhA mutations in various study areas.

Keywords

drug-resistant TB; heteroresistance; mutations; spoligotyping

Subject

Medicine and Pharmacology, Epidemiology and Infectious Diseases

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comments (1)

Comment 1
Received: 9 June 2023
Commenter: LINDIWE FAYE
Commenter's Conflict of Interests: Author
Comment: -
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