Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Hypoxia-Induced Cardiomyocyte Apoptosis Exacerbates Cardiomyocyte Hypertrophy and Myocardial Injury through p38 MAPK Pathway in SD Rats

Version 1 : Received: 13 February 2023 / Approved: 16 February 2023 / Online: 16 February 2023 (07:21:53 CET)

How to cite: Li, X.; Pu, Z.; Xu, G.; Yang, Y.; Cui, Y.; Zhou, X.; Wang, C.; Zhong, Z.; Zhou, S.; Yi, J.; Shan, F.; Yang, C.; Jiao, L.; Chen, D.; Huang, J. Hypoxia-Induced Cardiomyocyte Apoptosis Exacerbates Cardiomyocyte Hypertrophy and Myocardial Injury through p38 MAPK Pathway in SD Rats. Preprints 2023, 2023020276. https://doi.org/10.20944/preprints202302.0276.v1 Li, X.; Pu, Z.; Xu, G.; Yang, Y.; Cui, Y.; Zhou, X.; Wang, C.; Zhong, Z.; Zhou, S.; Yi, J.; Shan, F.; Yang, C.; Jiao, L.; Chen, D.; Huang, J. Hypoxia-Induced Cardiomyocyte Apoptosis Exacerbates Cardiomyocyte Hypertrophy and Myocardial Injury through p38 MAPK Pathway in SD Rats. Preprints 2023, 2023020276. https://doi.org/10.20944/preprints202302.0276.v1

Abstract

Right ventricular remodeling and its function are closely related to the symptom severity and survival of hypoxia pulmonary hypertension, but molecular mechanisms of cardiomyocyte hypertrophy and myocardial injury remain unclear. It evaluated the cardiac function (by right cardiac catheterization to measure right ventricular systolic pressure and mean pulmonary artery pressure), myocardial tissue morphology(Haematoxylin and Eosin), myocardial hypertrophy (Wheat Germ Agglutinin Staining), then RNA sequencing was applied to explore the mechanism, which results were verified by western blot in final. Hypoxia developed pulmonary hypertension, right ventricular diastolic and systolic functions were enhanced in rats, such as the mean pulmonary artery pressure, systolic pressure of pulmonary artery, the max pressure of right ventricular, the mean right ventricular pressure, and the heart rate was significantly higher than that in control. We found that Hif-1 signaling pathway and p38-MAPK signaling pathway have been activated by hypoxia, and cardiomyocyte apoptosis also aggravated in the right ventricular, which might be one cause of cardiomyocyte hypertrophy and myocardial injury, and targeted intervention might be one potential prevention for cardiomyocyte hypertrophy and myocardial injury induced by hypoxia.

Keywords

Hypoxia; p38; Apoptosis; Cardiomyocyte Hypertrophy; Myocardial Injury

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

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