Bucciarelli, A.; Vighi, N.; Bossi, A.M.; Grigolo, B.; Maniglio, D. Porous Thermoplastic Molded Regenerated Silk Crosslinked by the Addition of Citric Acid. Materials2023, 16, 1535.
Bucciarelli, A.; Vighi, N.; Bossi, A.M.; Grigolo, B.; Maniglio, D. Porous Thermoplastic Molded Regenerated Silk Crosslinked by the Addition of Citric Acid. Materials 2023, 16, 1535.
Bucciarelli, A.; Vighi, N.; Bossi, A.M.; Grigolo, B.; Maniglio, D. Porous Thermoplastic Molded Regenerated Silk Crosslinked by the Addition of Citric Acid. Materials2023, 16, 1535.
Bucciarelli, A.; Vighi, N.; Bossi, A.M.; Grigolo, B.; Maniglio, D. Porous Thermoplastic Molded Regenerated Silk Crosslinked by the Addition of Citric Acid. Materials 2023, 16, 1535.
Abstract
Thermoplastic molded regenerated silk was proposed as structural material in tissue engineering applications, mainly for application in bone. The protocol allows to obtain a compact non-porous material with a compression modulus in the order of a Giga Pascal starting from silk fibroin by compressing a silk fibroin lyophilized sponge of powder in mold at temperature higher than the glass transition temperature (Tg). The main purpose of the produced resin was the osteofixation and other structural applications in which the lack of porosity was not an issue. In this work, we introduced the use of citric acid in the thermoplastic molding protocol of SF to obtain a porosity inside the structural material. In addition to the previously developed protocols the addition of citric acid allowed us to obtain a structural material. The CA powder during the compression acted as a template for the pore formation. In addition, the CA was able to effectively crosslink the SF chain improving the mechanical strength. This effect was proved both evaluating the compression modulus and by studying the spectra obtained by Fourier infrared spectroscopy (FTIR). This protocol may be applied in the near future to the production of a structural bone scaffold.
Keywords
Bone Tissue Engineering; Bone Scaffold; Citric Acid; Silk Resin; Compression Molding
Subject
Chemistry and Materials Science, Biomaterials
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
