Version 1
: Received: 2 January 2023 / Approved: 4 January 2023 / Online: 4 January 2023 (01:27:09 CET)
Version 2
: Received: 21 January 2023 / Approved: 22 January 2023 / Online: 22 January 2023 (06:50:19 CET)
Rossi, F.; Del Matto, I.; Saletti, M.A.; Ricchiuti, L.; Tucci, P.; Marino, L. Recent Trends of Antibiotic Resistance in Staphylococcus Aureus Causing Clinical Mastitis in Dairy Herds in Abruzzo and Molise Regions, Italy. Antibiotics 2023, 12, 430, doi:10.3390/antibiotics12030430.
Rossi, F.; Del Matto, I.; Saletti, M.A.; Ricchiuti, L.; Tucci, P.; Marino, L. Recent Trends of Antibiotic Resistance in Staphylococcus Aureus Causing Clinical Mastitis in Dairy Herds in Abruzzo and Molise Regions, Italy. Antibiotics 2023, 12, 430, doi:10.3390/antibiotics12030430.
Rossi, F.; Del Matto, I.; Saletti, M.A.; Ricchiuti, L.; Tucci, P.; Marino, L. Recent Trends of Antibiotic Resistance in Staphylococcus Aureus Causing Clinical Mastitis in Dairy Herds in Abruzzo and Molise Regions, Italy. Antibiotics 2023, 12, 430, doi:10.3390/antibiotics12030430.
Rossi, F.; Del Matto, I.; Saletti, M.A.; Ricchiuti, L.; Tucci, P.; Marino, L. Recent Trends of Antibiotic Resistance in Staphylococcus Aureus Causing Clinical Mastitis in Dairy Herds in Abruzzo and Molise Regions, Italy. Antibiotics 2023, 12, 430, doi:10.3390/antibiotics12030430.
Abstract
This study was aimed to examine the recent trends of antibiotic resistance (AR) prevalence in Staphylococcus aureus isolated from milk of animals with clinical mastitis in areas of the Abruzzo and Molise regions in central Italy. Fifty-four S. aureus isolates could be obtained from routine testing for clinical mastitis agents carried out in the author institution in years 2021 and 2022. These were analyzed for phenotypic resistance to eight antibiotics recommended for testing by European norms and belonging to the antibiotic classes used for mastitis treatment in milk producing animals. Moreover, the presence of 14 transferable genetic determinants encoding resistance to the same antibiotics was analyzed by qPCR tests developed in this study. Phenotypic resistance to non-β-lactams was infrequent, with only one 2022 isolate resistant to clindamycin. However, low level resistance to the β-lactam cefoxitin was observed in 59.2% isolates in both years making these isolates classifiable as methicillin resistant. The AR genotypes detected were blaZ gene (50% 2021 isolates and 44.4% 2022 isolates), ermC/T- aphA3-blaZ (one 2021 isolate), ant6-ermC/T-aphA3-blaZ (one 2021 isolate), ermB-blaZ (one 2022 isolate) and mecA-mph(one 2022 isolate). An interview to the veterinarians who conferred the samples, regarding antimicrobials prescribed for mastitis treatment and criteria of usage, indicated a possible causal relation with the AR test results. The low prevalence of AR genotypes, not increasing in time, most probably reflecting the reported management of antibiotic therapies in farms. However, the frequently observed cefoxitin resistance needs to be explained genotypically, further monitored and limited by modifying antibiotic usage practices. The identification of a mecA positive isolate in 2022 suggests to investigate further if this genotype is emerging locally.
Keywords
Staphylococcus aureus; clinical mastitis; antibiotic resistance (AR) prevalence; AR phenotype; AR genotype; recent trend
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Commenter: Franca Rossi
Commenter's Conflict of Interests: Author