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Involvement of the Small ncRNAome in Fresh Peripheral Blood Mononuclear Cells of Human T-cell Leukemia Virus Type I- Associated Myelopathy/Tropical Spastic Paraparesis Patients and Viral Carriers
Souza, D.R.V.; Pessôa, R.; Nukui, Y.; Pereira, J.; Marcusso, R.N.; Penalva de Oliveira, A.C.; Casseb, J.; Duarte, A.J.D.S.; Clissa, P.B.; Sanabani, S.S. Involvement of the Small ncRNAome in Fresh Peripheral Blood Mononuclear Cells of Human T-cell Leukemia Virus Type I- Associated Myelopathy/Tropical Spastic Paraparesis Patients and Viral Carriers. Preprints2022, 2022120401. https://doi.org/10.20944/preprints202212.0401.v1
APA Style
Souza, D.R.V., Pessôa, R., Nukui, Y., Pereira, J., Marcusso, R.N., Penalva de Oliveira, A.C., Casseb, J., Duarte, A.J.D.S., Clissa, P.B., & Sanabani, S.S. (2022). Involvement of the Small ncRNAome in Fresh Peripheral Blood Mononuclear Cells of Human T-cell Leukemia Virus Type I- Associated Myelopathy/Tropical Spastic Paraparesis Patients and Viral Carriers. Preprints. https://doi.org/10.20944/preprints202212.0401.v1
Chicago/Turabian Style
Souza, D.R.V., Patricia Bianca Clissa and Sabri Saeed Sanabani. 2022 "Involvement of the Small ncRNAome in Fresh Peripheral Blood Mononuclear Cells of Human T-cell Leukemia Virus Type I- Associated Myelopathy/Tropical Spastic Paraparesis Patients and Viral Carriers" Preprints. https://doi.org/10.20944/preprints202212.0401.v1
Abstract
Human T-lymphotropic virus type 1 (HTLV-1)–associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a slowly progressive spinal cord disorder with no effective treatment. There is much of interest in developing potential biomarkers for predicting the pathogenesis of HAM/TSP disorder. This study used Illumina massive parallel sequencing (MPS) technology to assess the cellular global noncoding RNAome expression profile in HAM/TSP patients (n = 10), asymptomatic HTLV-1 infected carriers (ASP, n = 8), and a second group of healthy controls (n = 5). Using various bioinformatics tools, the sRNA MPS reads were aligned, annotated, and profiled. There were 251 known and 50 potential novel sRNAs among the 402 detected sRNAs in the HAM and ASP groups versus the HC group. Sixty-eight known sRNAs were found to be significantly different between the ASP and HAM groups. In HAM vs ASP subjects, 88 mature miRNAs were downregulated. Three of these miRs (hsa-miR-185-5p, 32-5p, and 192-5p) have the potential to be used as biomarkers for predicting the pathogenesis of HAM/TSP. The top seven deregulated miRS target genes were linked to a variety of biological processes and molecular functions. Relevant reactome pathways to our findings provide a rich source of data and an opportunity to further understand sRNA regulation and function in HTLV-1 pathophysiology.
Keywords
small RNA; HTLV-1; HAM/ TSP; massive parallel sequencing
Subject
Medicine and Pharmacology, Pathology and Pathobiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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